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J. Bacteriol., Apr 1996, 2440-2444, Vol 178, No. 8
Copyright © 1996, American Society for Microbiology

Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum

G Weidner and G Sawers
Department of Microbiology and Genetics, University of Munich, Germany.

Formate is the major source of C1 units in many species of the genus Clostridium. In this study we have cloned and characterized the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum. The genetic and transcriptional organizations of the genes and the high level of homology exhibited by the respective gene products to their Escherichia coli counterparts indicate strong evolutionary conservation of these enzymes.


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