This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coffman, J. A.
Right arrow Articles by Cooper, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coffman, J. A.
Right arrow Articles by Cooper, T. G.

 Previous Article  |  Next Article 

J. Bacteriol., 06 1997, 3416-3429, Vol 179, No. 11
Copyright © 1997, American Society for Microbiology

Cross regulation of four GATA factors that control nitrogen catabolic gene expression in Saccharomyces cerevisiae

JA Coffman, R Rai, DM Loprete, T Cunningham, V Svetlov and TG Cooper
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163, USA.

Nitrogen catabolic gene expression in Saccharomyces cerevisiae has been reported to be regulated by three GATA family proteins, the positive regulators Gln3p and Gat1p/Nil1p and the negative regulator Dal80p/Uga43p. We show here that a fourth member of the yeast GATA family, the Dal80p homolog Deh1p, also negatively regulates expression of some, but not all, nitrogen catabolic genes, i.e., GAP1, DAL80, and UGA4 expression increases in a deh1 delta mutant. Consistent with Deh1p regulation of these genes is the observation that Deh1p forms specific DNA-protein complexes with GATAA-containing UGA4 and GAP1 promoter fragments in electrophoretic mobility shift assays. Deh1p function is demonstrable, however, only when a repressive nitrogen source such as glutamine is present; deh1 delta mutants exhibit no detectable phenotype with a poor nitrogen source such as proline. Our experiments also demonstrate that GATA factor gene expression is highly regulated by the GATA factors themselves in an interdependent manner. DAL80 expression is Gln3p and Gat1p dependent and Dal80p regulated. Moreover, Gln3p and Dal80p bind to DAL80 promoter fragments. In turn, GAT1 expression is Gln3p dependent and Dal80p regulated but is not autogenously regulated like DAL80. DEH1 expression is largely Gln3p independent, modestly Gat1p dependent, and most highly regulated by Dal80p. Paradoxically, the high-level DEH1 expression observed in a dal80::hisG disruption mutant is highly sensitive to nitrogen catabolite repression.


This article has been cited by other articles:

  • Georis, I., Feller, A., Vierendeels, F., Dubois, E. (2009). The Yeast GATA Factor Gat1 Occupies a Central Position in Nitrogen Catabolite Repression-Sensitive Gene Activation. Mol. Cell. Biol. 29: 3803-3815 [Abstract] [Full Text]  
  • Georis, I., Feller, A., Tate, J. J., Cooper, T. G., Dubois, E. (2009). Nitrogen Catabolite Repression-Sensitive Transcription as a Readout of Tor Pathway Regulation: The Genetic Background, Reporter Gene and GATA Factor Assayed Determine the Outcomes. Genetics 181: 861-874 [Abstract] [Full Text]  
  • de la Riva, L., Badia, J., Aguilar, J., Bender, R. A., Baldoma, L. (2008). The hpx Genetic System for Hypoxanthine Assimilation as a Nitrogen Source in Klebsiella pneumoniae: Gene Organization and Transcriptional Regulation. J. Bacteriol. 190: 7892-7903 [Abstract] [Full Text]  
  • Wong, K. H., Hynes, M. J., Davis, M. A. (2008). Recent Advances in Nitrogen Regulation: a Comparison between Saccharomyces cerevisiae and Filamentous Fungi. Eukaryot Cell 7: 917-925 [Full Text]  
  • Georis, I., Tate, J. J., Cooper, T. G., Dubois, E. (2008). Tor Pathway Control of the Nitrogen-responsive DAL5 Gene Bifurcates at the Level of Gln3 and Gat1 Regulation in Saccharomyces cerevisiae. J. Biol. Chem. 283: 8919-8929 [Abstract] [Full Text]  
  • Luzzani, C., Cardillo, S. B., Bermudez Moretti, M., Correa Garcia, S. (2007). New insights into the regulation of the Saccharomyces cerevisiae UGA4 gene: two parallel pathways participate in carbon-regulated transcription. Microbiology 153: 3677-3684 [Abstract] [Full Text]  
  • Godard, P., Urrestarazu, A., Vissers, S., Kontos, K., Bontempi, G., van Helden, J., Andre, B. (2007). Effect of 21 Different Nitrogen Sources on Global Gene Expression in the Yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 27: 3065-3086 [Abstract] [Full Text]  
  • Garcia-Salcedo, R., Casamayor, A., Ruiz, A., Gonzalez, A., Prista, C., Loureiro-Dias, M. C., Ramos, J., Arino, J. (2006). Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii.. Eukaryot Cell 5: 1388-1398 [Abstract] [Full Text]  
  • Abdel-Sater, F., Iraqui, I., Urrestarazu, A., Andre, B. (2004). The External Amino Acid Signaling Pathway Promotes Activation of Stp1 and Uga35/Dal81 Transcription Factors for Induction of the AGP1 Gene in Saccharomyces cerevisiae. Genetics 166: 1727-1739 [Abstract] [Full Text]  
  • Saxena, D., Kannan, K. B., Brandriss, M. C. (2003). Rapamycin Treatment Results in GATA Factor-Independent Hyperphosphorylation of the Proline Utilization Pathway Activator in Saccharomyces cerevisiae. Eukaryot Cell 2: 552-559 [Abstract] [Full Text]  
  • Nakagawa, Y., Sugioka, S., Kaneko, Y., Harashima, S. (2001). O2R, a Novel Regulatory Element Mediating Rox1p-Independent O2 and Unsaturated Fatty Acid Repression of OLE1 in Saccharomyces cerevisiae. J. Bacteriol. 183: 745-751 [Abstract] [Full Text]  
  • Cunningham, T. S., Rai, R., Cooper, T. G. (2000). The Level of DAL80 Expression Down-Regulates GATA Factor-Mediated Transcription in Saccharomyces cerevisiae. J. Bacteriol. 182: 6584-6591 [Abstract] [Full Text]  
  • Komeili, A., Wedaman, K. P., O'Shea, E. K., Powers, T. (2000). Mechanism of Metabolic Control: Target of Rapamycin Signaling Links Nitrogen Quality to the Activity of the Rtg1 and Rtg3 Transcription Factors. JCB 151: 863-878 [Abstract] [Full Text]  
  • Zadra, I., Abt, B., Parson, W., Haas, H. (2000). xylP Promoter-Based Expression System and Its Use for Antisense Downregulation of the Penicillium chrysogenum Nitrogen Regulator NRE. Appl. Environ. Microbiol. 66: 4810-4816 [Abstract] [Full Text]  
  • Cunningham, T. S., Andhare, R., Cooper, T. G. (2000). Nitrogen Catabolite Repression of DAL80 Expression Depends on the Relative Levels of Gat1p and Ure2p Production in Saccharomyces cerevisiae. J. Biol. Chem. 275: 14408-14414 [Abstract] [Full Text]  
  • Scott, S., Dorrington, R., Svetlov, V., Beeser, A. E., Distler, M., Cooper, T. G. (2000). Functional Domain Mapping and Subcellular Distribution of Dal82p in Saccharomyces cerevisiae. J. Biol. Chem. 275: 7198-7204 [Abstract] [Full Text]  
  • Huang, H. L., Brandriss, M. C. (2000). The Regulator of the Yeast Proline Utilization Pathway Is Differentially Phosphorylated in Response to the Quality of the Nitrogen Source. Mol. Cell. Biol. 20: 892-899 [Abstract] [Full Text]  
  • Hardwick, J. S., Kuruvilla, F. G., Tong, J. K., Shamji, A. F., Schreiber, S. L. (1999). Rapamycin-modulated transcription defines the subset of nutrient-sensitive signaling pathways directly controlled by the Tor proteins. Proc. Natl. Acad. Sci. USA 96: 14866-14870 [Abstract] [Full Text]  
  • Park, H.-D., Scott, S., Rai, R., Dorrington, R., Cooper, T. G. (1999). Synergistic Operation of the CAR2 (Ornithine Transaminase) Promoter Elements in Saccharomyces cerevisiae. J. Bacteriol. 181: 7052-7064 [Abstract] [Full Text]  
  • Edskes, H. K., Hanover, J. A., Wickner, R. B. (1999). Mks1p Is a Regulator of Nitrogen Catabolism Upstream of Ure2p in Saccharomyces cerevisiae. Genetics 153: 585-594 [Abstract] [Full Text]  
  • Rai, R., Daugherty, J. R., Cunningham, T. S., Cooper, T. G. (1999). Overlapping Positive and Negative GATA Factor Binding Sites Mediate Inducible DAL7 Gene Expression in Saccharomyces cerevisiae. J. Biol. Chem. 274: 28026-28034 [Abstract] [Full Text]  
  • Small, A. J., Hynes, M. J., Davis, M. A. (1999). The TamA Protein Fused to a DNA-Binding Domain Can Recruit AreA, the Major Nitrogen Regulatory Protein, to Activate Gene Expression in Aspergillus nidulans. Genetics 153: 95-105 [Abstract] [Full Text]  
  • Klasson, H., Fink, G. R., Ljungdahl, P. O. (1999). Ssy1p and Ptr3p Are Plasma Membrane Components of a Yeast System That Senses Extracellular Amino Acids. Mol. Cell. Biol. 19: 5405-5416 [Abstract] [Full Text]  
  • Iraqui, I., Vissers, S., Andre, B., Urrestarazu, A. (1999). Transcriptional Induction by Aromatic Amino Acids in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 3360-3371 [Abstract] [Full Text]  
  • Beeser, A. E., Cooper, T. G. (1999). Control of Nitrogen Catabolite Repression Is Not Affected by the tRNAGln-CUU Mutation, Which Results in Constitutive Pseudohyphal Growth of Saccharomyces cerevisiae. J. Bacteriol. 181: 2472-2476 [Abstract] [Full Text]  
  • Svetlov, V. V., Cooper, T. G. (1998). The Saccharomyces cerevisiae GATA Factors Dal80p and Deh1p Can Form Homo- and Heterodimeric Complexes. J. Bacteriol. 180: 5682-5688 [Abstract] [Full Text]  
  • Valenzuela, L., Ballario, P., Aranda, C., Filetici, P., González, A. (1998). Regulation of Expression of GLT1, the Gene Encoding Glutamate Synthase in Saccharomyces cerevisiae. J. Bacteriol. 180: 3533-3540 [Abstract] [Full Text]  
  • Cox, K. H., Rai, R., Distler, M., Daugherty, J. R., Coffman, J. A., Cooper, T. G. (2000). Saccharomyces cerevisiae GATA Sequences Function as TATA Elements during Nitrogen Catabolite Repression and When Gln3p Is Excluded from the Nucleus by Overproduction of Ure2p. J. Biol. Chem. 275: 17611-17618 [Abstract] [Full Text]  
  • Bertram, P. G., Choi, J. H., Carvalho, J., Ai, W., Zeng, C., Chan, T.-F., Zheng, X. F. S. (2000). Tripartite Regulation of Gln3p by TOR, Ure2p, and Phosphatases. J. Biol. Chem. 275: 35727-35733 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»