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J. Bacteriol., 07 1997, 4285-4291, Vol 179, No. 13
Copyright © 1997, American Society for Microbiology

Characterization of cp18, a naturally truncated member of the cp32 family of Borrelia burgdorferi plasmids

B Stevenson, S Casjens, R van Vugt, SF Porcella, K Tilly, JL Bono and P Rosa
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA. brian_stevenson@nih.gov

We have mapped the genes encoding the antigenic lipoproteins OspE and OspF to an approximately 18-kb circular plasmid in Borrelia burgdorferi N40. Sequencing and restriction mapping have revealed that this plasmid, cp18, is homologous to an 18-kb region of the cp32 circular plasmids found in the Lyme disease spirochetes. Our data show that cp18 may have arisen from an ancestral cp32 plasmid by deletion of a 14-kb region of DNA, indicating that a significant portion of the cp32 plasmid is not essential in cis for plasmid maintenance. These findings suggest that a relatively small recombinant plasmid capable of being stably maintained in B. burgdorferi could be constructed from a cp32 plasmid.


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