This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cornillot, E.
Right arrow Articles by Soucaille, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cornillot, E.
Right arrow Articles by Soucaille, P.

 Previous Article  |  Next Article 

J. Bacteriol., Sep 1997, 5442-5447, Vol 179, No. 17
Copyright © 1997, American Society for Microbiology

The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain

E Cornillot, RV Nair, ET Papoutsakis and P Soucaille
INSA, Centre de Bioingenierie Gilbert Durand, Complexe Scientifique deRangueil, Toulouse, France.

Degeneration is the process whereby Clostridium acetobutylicum ATCC 824 loses the capacity to produce acetone and butanol after repeated vegetative transfers or in continuous culture. Two degenerate mutants (M5 and DG1) of C. acetobutylicum ATCC 824 do not contain the four genes (ctfA, ctfB, adc, and aad) for acetone and butanol formation. Strain ATCC 824 contains a 210-kb plasmid (pSOL1) which is absent in M5 and DG1. pSOL1 carries the four acetone and butanol formation genes. A restriction map of pSOL1 was constructed by using ApaI, SmaI, SstII, and NarI digestions. M5 and DG1 could be complemented for acetone and butanol formation by expressing the corresponding genes (ctfA, ctfB, and adc for acetone; aad for butanol) on the plasmid. Degeneration of this strain thus appears to be the result of pSOL1 loss.


This article has been cited by other articles:

  • Tracy, B. P., Gaida, S. M., Papoutsakis, E. T. (2008). Development and Application of Flow-Cytometric Techniques for Analyzing and Sorting Endospore-Forming Clostridia. Appl. Environ. Microbiol. 74: 7497-7506 [Abstract] [Full Text]  
  • Paredes, C. J., Senger, R. S., Spath, I. S., Borden, J. R., Sillers, R., Papoutsakis, E. T. (2007). A General Framework for Designing and Validating Oligomer-Based DNA Microarrays and Its Application to Clostridium acetobutylicum. Appl. Environ. Microbiol. 73: 4631-4638 [Abstract] [Full Text]  
  • Borden, J. R., Papoutsakis, E. T. (2007). Dynamics of Genomic-Library Enrichment and Identification of Solvent Tolerance Genes for Clostridium acetobutylicum. Appl. Environ. Microbiol. 73: 3061-3068 [Abstract] [Full Text]  
  • Gonzalez-Pajuelo, M., Meynial-Salles, I., Mendes, F., Soucaille, P., Vasconcelos, I. (2006). Microbial Conversion of Glycerol to 1,3-Propanediol: Physiological Comparison of a Natural Producer, Clostridium butyricum VPI 3266, and an Engineered Strain, Clostridium acetobutylicum DG1(pSPD5). Appl. Environ. Microbiol. 72: 96-101 [Abstract] [Full Text]  
  • Alsaker, K. V., Papoutsakis, E. T. (2005). Transcriptional Program of Early Sporulation and Stationary-Phase Events in Clostridium acetobutylicum. J. Bacteriol. 187: 7103-7118 [Abstract] [Full Text]  
  • Scotcher, M. C., Rudolph, F. B., Bennett, G. N. (2005). Expression of abrB310 and sinR, and Effects of Decreased abrB310 Expression on the Transition from Acidogenesis to Solventogenesis, in Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 71: 1987-1995 [Abstract] [Full Text]  
  • Scotcher, M. C., Bennett, G. N. (2005). SpoIIE Regulates Sporulation but Does Not Directly Affect Solventogenesis in Clostridium acetobutylicum ATCC 824. J. Bacteriol. 187: 1930-1936 [Abstract] [Full Text]  
  • Zhao, Y., Tomas, C. A., Rudolph, F. B., Papoutsakis, E. T., Bennett, G. N. (2005). Intracellular Butyryl Phosphate and Acetyl Phosphate Concentrations in Clostridium acetobutylicum and Their Implications for Solvent Formation. Appl. Environ. Microbiol. 71: 530-537 [Abstract] [Full Text]  
  • Tomas, C. A., Beamish, J., Papoutsakis, E. T. (2004). Transcriptional Analysis of Butanol Stress and Tolerance in Clostridium acetobutylicum. J. Bacteriol. 186: 2006-2018 [Abstract] [Full Text]  
  • Tomas, C. A., Welker, N. E., Papoutsakis, E. T. (2003). Overexpression of groESL in Clostridium acetobutylicum Results in Increased Solvent Production and Tolerance, Prolonged Metabolism, and Changes in the Cell's Transcriptional Program. Appl. Environ. Microbiol. 69: 4951-4965 [Abstract] [Full Text]  
  • Tomas, C. A., Alsaker, K. V., Bonarius, H. P. J., Hendriksen, W. T., Yang, H., Beamish, J. A., Paredes, C. J., Papoutsakis, E. T. (2003). DNA Array-Based Transcriptional Analysis of Asporogenous, Nonsolventogenic Clostridium acetobutylicum Strains SKO1 and M5. J. Bacteriol. 185: 4539-4547 [Abstract] [Full Text]  
  • Zhao, Y., Hindorff, L. A., Chuang, A., Monroe-Augustus, M., Lyristis, M., Harrison, M. L., Rudolph, F. B., Bennett, G. N. (2003). Expression of a Cloned Cyclopropane Fatty Acid Synthase Gene Reduces Solvent Formation in Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 69: 2831-2841 [Abstract] [Full Text]  
  • Tummala, S. B., Welker, N. E., Papoutsakis, E. T. (2003). Design of Antisense RNA Constructs for Downregulation of the Acetone Formation Pathway of Clostridium acetobutylicum. J. Bacteriol. 185: 1923-1934 [Abstract] [Full Text]  
  • Watrous, M. M., Clark, S., Kutty, R., Huang, S., Rudolph, F. B., Hughes, J. B., Bennett, G. N. (2003). 2,4,6-Trinitrotoluene Reduction by an Fe-Only Hydrogenase in Clostridium acetobutylicum. Appl. Environ. Microbiol. 69: 1542-1547 [Abstract] [Full Text]  
  • Yang, H., Haddad, H., Tomas, C., Alsaker, K., Papoutsakis, E. T. (2003). A segmental nearest neighbor normalization and gene identification method gives superior results for DNA-array analysis. Proc. Natl. Acad. Sci. USA 100: 1122-1127 [Abstract] [Full Text]  
  • Harris, L. M., Welker, N. E., Papoutsakis, E. T. (2002). Northern, Morphological, and Fermentation Analysis of spo0A Inactivation and Overexpression in Clostridium acetobutylicum ATCC 824. J. Bacteriol. 184: 3586-3597 [Abstract] [Full Text]  
  • Thormann, K., Feustel, L., Lorenz, K., Nakotte, S., Durre, P. (2002). Control of Butanol Formation in Clostridium acetobutylicum by Transcriptional Activation. J. Bacteriol. 184: 1966-1973 [Abstract] [Full Text]  
  • Fontaine, L., Meynial-Salles, I., Girbal, L., Yang, X., Croux, C., Soucaille, P. (2002). Molecular Characterization and Transcriptional Analysis of adhE2, the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824. J. Bacteriol. 184: 821-830 [Abstract] [Full Text]  
  • Nolling, J., Breton, G., Omelchenko, M. V., Makarova, K. S., Zeng, Q., Gibson, R., Lee, H. M., Dubois, J., Qiu, D., Hitti, J., GTC Sequencing Center Production, , Finishing, and Bioinformatics Teams, , Wolf, Y. I., Tatusov, R. L., Sabathe, F., Doucette-Stamm, L., Soucaille, P., Daly, M. J., Bennett, G. N., Koonin, E. V., Smith, D. R. (2001). Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum. J. Bacteriol. 183: 4823-4838 [Abstract] [Full Text]  
  • Chen, C.-K., Blaschek, H. P. (1999). Effect of Acetate on Molecular and Physiological Aspects of Clostridium beijerinckii NCIMB 8052 Solvent Production and Strain Degeneration. Appl. Environ. Microbiol. 65: 499-505 [Abstract] [Full Text]  
  • Nair, R. V., Green, E. M., Watson, D. E., Bennett, G. N., Papoutsakis, E. T. (1999). Regulation of the sol Locus Genes for Butanol and Acetone Formation in Clostridium acetobutylicum ATCC 824 by a Putative Transcriptional Repressor. J. Bacteriol. 181: 319-330 [Abstract] [Full Text]  
  • Evans, V. J., Liyanage, H., Ravagnani, A., Young, M., Kashket, E. R. (1998). Truncation of Peptide Deformylase Reduces the Growth Rate and Stabilizes Solvent Production in Clostridium beijerinckii NCIMB 8052. Appl. Environ. Microbiol. 64: 1780-1785 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»