This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Henriques, A. O. Articles by Moran, C. P. Search for Related Content PubMed PubMed Citation Articles by Henriques, A. O. Articles by Moran, C. P., Jr

 Previous Article  |  Next Article 

J. Bacteriol., Jan 1997, 389-398, Vol 179, No. 2
Copyright © 1997, American Society for Microbiology

cse15, cse60, and csk22 are new members of mother-cell-specific sporulation regulons in Bacillus subtilis

AO Henriques, EM Bryan, BW Beall and CP Moran Jr
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requirement for sigmaE. Maximal expression of both cse15 and cse60 further depended on the DNA-binding protein SpoIIID. Primer extension results revealed -10 and -35 sequences upstream of the cse15 and cse60 coding sequences very similar to those utilized by sigmaE-containing RNA polymerase. Alignment of these and other regulatory regions led to a revised consensus sequence for sigmaE-dependent promoters. A third transcriptional unit, designated csk22, was localized at approximately 173 degrees on the chromosome. Transcription of csk22 was activated at h 4 of sporulation, required the late mother-cell regulator sigmaK, and was repressed by the GerE protein. Sequences in the csk22 promoter region were similar to those of other sigmaK-dependent promoters. The cse60 locus was deduced to encode an acidic product of only 60 residues. A 37.6-kDa protein apparently encoded by cse15 was weakly related to the heavy chain of myosins, as well as to other myosin-like proteins, and is predicted to contain a central, 100 residue-long coiled-coil domain. Finally, csk22 is inferred to encode a 18.2-kDa hydrophobic product with five possible membrane-spanning helices, which could function as a transporter.

This article has been cited by other articles:

• Real, G., Henriques, A. O. (2006). Localization of the Bacillus subtilis murB Gene within the dcw Cluster Is Important for Growth and Sporulation.. J. Bacteriol. 188: 1721-1732 [Abstract] [Full Text]  
• Real, G., Pinto, S. M., Schyns, G., Costa, T., Henriques, A. O., Moran, C. P. Jr. (2005). A Gene Encoding a Holin-Like Protein Involved in Spore Morphogenesis and Spore Germination in Bacillus subtilis. J. Bacteriol. 187: 6443-6453 [Abstract] [Full Text]  
• Albano, M., Smits, W. K., Ho, L. T. Y., Kraigher, B., Mandic-Mulec, I., Kuipers, O. P., Dubnau, D. (2005). The Rok Protein of Bacillus subtilis Represses Genes for Cell Surface and Extracellular Functions. J. Bacteriol. 187: 2010-2019 [Abstract] [Full Text]  
• Steil, L., Serrano, M., Henriques, A. O., Volker, U. (2005). Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis. Microbiology 151: 399-420 [Abstract] [Full Text]  
• Hilbert, D. W., Piggot, P. J. (2003). Novel spoIIE Mutation That Causes Uncompartmentalized {sigma}F Activation in Bacillus subtilis. J. Bacteriol. 185: 1590-1598 [Abstract] [Full Text]  
• Rudner, D. Z., Pan, Q., Losick, R. M. (2002). Evidence that subcellular localization of a bacterial membrane protein is achieved by diffusion and capture. Proc. Natl. Acad. Sci. USA 99: 8701-8706 [Abstract] [Full Text]  
• Kodama, T., Takamatsu, H., Asai, K., Kobayashi, K., Ogasawara, N., Watabe, K. (1999). The Bacillus subtilis yaaH Gene Is Transcribed by SigE RNA Polymerase during Sporulation, and Its Product Is Involved in Germination of Spores. J. Bacteriol. 181: 4584-4591 [Abstract] [Full Text]  
• Wade, K. H., Schyns, G., Opdyke, J. A., Moran, C. P. Jr. (1999). A Region of sigma K Involved in Promoter Activation by GerE in Bacillus subtilis. J. Bacteriol. 181: 4365-4373 [Abstract] [Full Text]  
• Ichikawa, H., Halberg, R., Kroos, L. (1999). Negative Regulation by the Bacillus subtilis GerE Protein. J. Biol. Chem. 274: 8322-8327 [Abstract] [Full Text]