This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kimura, Y. Articles by Sato, M. Search for Related Content PubMed PubMed Citation Articles by Kimura, Y. Articles by Sato, M.

 Previous Article  |  Next Article 

J. Bacteriol., 11 1997, 7098-7102, Vol 179, No. 22
Copyright © 1997, American Society for Microbiology

Propionyl coenzyme A carboxylase is required for development of Myxococcus xanthus

Y Kimura, R Sato, K Mimura and M Sato
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan. kimura@ag.kagawa-u.ac.jp

A dcm-1 mutant, obtained by transposon mutagenesis of Myxococcus xanthus, could aggregate and form mounds but was unable to sporulate under nutrient starvation. A sequence analysis of the site of insertion of the transposon showed that the insertion lies within the 3' end of a 1,572-bp open reading frame (ORF) designated the M. xanthus pccB ORF. The wild-type form of the M. xanthus pccB gene, obtained from a lambdaEMBL library of M. xanthus, shows extensive similarity to a beta subunit of propionyl coenzyme A (CoA) carboxylase, an alpha subunit of methylmalonyl-CoA decarboxylase, and a 12S subunit of transcarboxylase. In enzyme assays, extracts of the dcm-1 mutant were deficient in propionyl-CoA carboxylase activity. This enzyme catalyzes the ATP- dependent carboxylation of propionyl-CoA to yield methylmalonyl-CoA. The methylmalonyl-CoA rescued the dcm-1 mutant fruiting body and spore development. During development, the dcm-1 mutant cells also had reduced levels of long-chain fatty acids (C16 to C18) compared to wild- type cells.

This article has been cited by other articles:

• Youderian, P., Hartzell, P. L. (2006). Transposon Insertions of magellan-4 That Impair Social Gliding Motility in Myxococcus xanthus. Genetics 172: 1397-1410 [Abstract] [Full Text]  
• Julien, B., Shah, S. (2002). Heterologous Expression of Epothilone Biosynthetic Genes in Myxococcus xanthus. Antimicrob. Agents Chemother. 46: 2772-2778 [Abstract] [Full Text]  
• Kimura, Y., Miyake, R., Tokumasu, Y., Sato, M. (2000). Molecular Cloning and Characterization of Two Genes for the Biotin Carboxylase and Carboxyltransferase Subunits of Acetyl Coenzyme A Carboxylase in Myxococcus xanthus. J. Bacteriol. 182: 5462-5469 [Abstract] [Full Text]