This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Robichon, D.
Right arrow Articles by Hechard, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Robichon, D.
Right arrow Articles by Hechard, Y.

 Previous Article  |  Next Article 

J. Bacteriol., Dec 1997, 7591-7594, Vol 179, No. 23
Copyright © 1997, American Society for Microbiology

The rpoN (sigma54) gene from Listeria monocytogenes is involved in resistance to mesentericin Y105, an antibacterial peptide from Leuconostoc mesenteroides

D Robichon, E Gouin, M Debarbouille, P Cossart, Y Cenatiempo and Y Hechard
Laboratoire de Biologie Moleculaire, URA CNRS 1172, IBMIG, UFR Sciences, Poitiers, France.

To gain insight into the mode of action of mesentericin Y105, a bacteriocin bactericidal agent against Listeria monocytogenes, we undertook to identify the listerial factors mediating this susceptibility by using a genetic approach. Transposon mutants resistant to the bacteriocin were obtained. One of them corresponded to a transposon insertion in a gene (rpoN) encoding a putative protein (447 amino acids) with strong homologies to alternative transcriptional sigma54 factors, including that of Bacillus subtilis (38% identity). Complementation experiments with the wild-type rpoN gene demonstrated that the insertion in rpoN was responsible for the resistance phenotype in L. monocytogenes. Moreover, expression of the L. monocytogenes rpoN gene in an rpoN mutant strain of B. subtilis promoted transcription of a sigma54-dependent operon in the presence of the associated regulator. These results demonstrate that the L. monocytogenes rpoN gene encodes a new sigma54 factor.


This article has been cited by other articles:

  • Tessema, G. T., Moretro, T., Kohler, A., Axelsson, L., Naterstad, K. (2009). Complex Phenotypic and Genotypic Responses of Listeria monocytogenes Strains Exposed to the Class IIa Bacteriocin Sakacin P. Appl. Environ. Microbiol. 75: 6973-6980 [Abstract] [Full Text]  
  • Vu-Khac, H., Miller, K. W. (2009). Regulation of Mannose Phosphotransferase System Permease and Virulence Gene Expression in Listeria monocytogenes by the EIItMan Transporter. Appl. Environ. Microbiol. 75: 6671-6678 [Abstract] [Full Text]  
  • Xue, J., Miller, K. W. (2007). Regulation of the mpt Operon in Listeria innocua by the ManR Protein. Appl. Environ. Microbiol. 73: 5648-5652 [Abstract] [Full Text]  
  • Drider, D., Fimland, G., Hechard, Y., McMullen, L. M., Prevost, H. (2006). The Continuing Story of Class IIa Bacteriocins. Microbiol. Mol. Biol. Rev. 70: 564-582 [Abstract] [Full Text]  
  • Kazmierczak, M. J., Wiedmann, M., Boor, K. J. (2005). Alternative Sigma Factors and Their Roles in Bacterial Virulence. Microbiol. Mol. Biol. Rev. 69: 527-543 [Abstract] [Full Text]  
  • Xue, J., Hunter, I., Steinmetz, T., Peters, A., Ray, B., Miller, K. W. (2005). Novel Activator of Mannose-Specific Phosphotransferase System Permease Expression in Listeria innocua, Identified by Screening for Pediocin AcH Resistance. Appl. Environ. Microbiol. 71: 1283-1290 [Abstract] [Full Text]  
  • Wemekamp-Kamphuis, H. H., Wouters, J. A., de Leeuw, P. P. L. A., Hain, T., Chakraborty, T., Abee, T. (2004). Identification of Sigma Factor {sigma}B-Controlled Genes and Their Impact on Acid Stress, High Hydrostatic Pressure, and Freeze Survival in Listeria monocytogenes EGD-e. Appl. Environ. Microbiol. 70: 3457-3466 [Abstract] [Full Text]  
  • Arous, S., Buchrieser, C., Folio, P., Glaser, P., Namane, A., Hebraud, M., Hechard, Y. (2004). Global analysis of gene expression in an rpoN mutant of Listeria monocytogenes. Microbiology 150: 1581-1590 [Abstract] [Full Text]  
  • Katla, T., Naterstad, K., Vancanneyt, M., Swings, J., Axelsson, L. (2003). Differences in Susceptibility of Listeria monocytogenes Strains to Sakacin P, Sakacin A, Pediocin PA-1, and Nisin. Appl. Environ. Microbiol. 69: 4431-4437 [Abstract] [Full Text]  
  • Vadyvaloo, V., Hastings, J. W., van der Merwe, M. J., Rautenbach, M. (2002). Membranes of Class IIa Bacteriocin-Resistant Listeria monocytogenes Cells Contain Increased Levels of Desaturated and Short-Acyl-Chain Phosphatidylglycerols. Appl. Environ. Microbiol. 68: 5223-5230 [Abstract] [Full Text]  
  • Gravesen, A., Ramnath, M., Rechinger, K. B., Andersen, N., Jansch, L., Hechard, Y., Hastings, J. W., Knochel, S. (2002). High-level resistance to class IIa bacteriocins is associated with one general mechanism in Listeria monocytogenes. Microbiology 148: 2361-2369 [Abstract] [Full Text]  
  • Liu, S., Graham, J. E., Bigelow, L., Morse, P. D. II, Wilkinson, B. J. (2002). Identification of Listeria monocytogenes Genes Expressed in Response to Growth at Low Temperature. Appl. Environ. Microbiol. 68: 1697-1705 [Abstract] [Full Text]  
  • Dalet, K., Cenatiempo, Y., Cossart, P., Hechard, Y. (2001). A {sigma}54-dependent PTS permease of the mannose family is responsible for sensitivity of Listeria monocytogenes to mesentericin Y105. Microbiology 147: 3263-3269 [Abstract] [Full Text]  
  • Hechard, Y., Pelletier, C., Cenatiempo, Y., Frere, J. (2001). Analysis of {{sigma}}54-dependent genes in Enterococcus faecalis: a mannose PTS permease (EIIMan) is involved in sensitivity to a bacteriocin, mesentericin Y105. Microbiology 147: 1575-1580 [Abstract] [Full Text]  
  • Duffes, F., Jenoe, P., Boyaval, P. (2000). Use of Two-Dimensional Electrophoresis To Study Differential Protein Expression in Divercin V41-Resistant and Wild-Type Strains of Listeria monocytogenes. Appl. Environ. Microbiol. 66: 4318-4324 [Abstract] [Full Text]  
  • Ramnath, M., Beukes, M., Tamura, K., Hastings, J. W. (2000). Absence of a Putative Mannose-Specific Phosphotransferase System Enzyme IIAB Component in a Leucocin A-Resistant Strain of Listeria monocytogenes, as Shown by Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Appl. Environ. Microbiol. 66: 3098-3101 [Abstract] [Full Text]  
  • Gravesen, A., Warthoe, P., Knøchel, S., Thirstrup, K. (2000). Restriction fragment differential display of pediocin-resistant Listeria monocytogenes 412 mutants shows consistent overexpression of a putative {beta}-glucoside-specific PTS system. Microbiology 146: 1381-1389 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»