This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishiguro, J.
Right arrow Articles by Ribas, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishiguro, J.
Right arrow Articles by Ribas, J. C.

 Previous Article  |  Next Article 

J. Bacteriol., 12 1997, 7653-7662, Vol 179, No. 24
Copyright © 1997, American Society for Microbiology

cps1+, a Schizosaccharomyces pombe gene homolog of Saccharomyces cerevisiae FKS genes whose mutation confers hypersensitivity to cyclosporin A and papulacandin B

J Ishiguro, A Saitou, A Duran and JC Ribas
Department of Biology, Faculty of Science, Konan University, Okamoto, Kobe, Japan.

The Schizosaccharomyces pombe cps1-12 (for chlorpropham supersensitive) mutant strain was originally isolated as hypersensitive to the spindle poison isopropyl N-3-chlorophenyl carbamate (chlorpropham) (J. Ishiguro and Y. Uhara, Jpn. J. Genet. 67:97-109, 1992). We have found that the cps1-12 mutation also confers (i) hypersensitivity to the immunosuppressant cyclosporin A (CsA), (ii) hypersensitivity to the drug papulacandin B, which specifically inhibits 1,3-beta-D-glucan synthesis both in vivo and in vitro, and (iii) thermosensitive growth at 37 degrees C. Under any of these restrictive treatments, cells swell up and finally lyse. With an osmotic stabilizer, cells do not lyse, but at 37 degrees C they become multiseptated and multibranched. The cps1- 12 mutant, grown at a restrictive temperature, showed an increase in sensitivity to lysis by enzymatic cell wall degradation, in in vitro 1,3-beta-D-glucan synthase activity (173% in the absence of GTP in the reaction), and in cell wall biosynthesis (130% of the wild-type amount). Addition of Ca2+ suppresses hypersensitivity to papulacandin B and septation and branching phenotypes. All of these data suggest a relationship between the cps1+ gene and cell wall synthesis. A DNA fragment containing the cps1+ gene was cloned, and sequence analysis indicated that it encodes a predicted membrane protein of 1,729 amino acids with 15 to 16 transmembrane domains. S. pombe cps1p has overall 55% sequence identity with Fks1p or Fks2p, proposed to be catalytic or associated subunits of Saccharomyces cerevisiae 1,3-beta-D-glucan synthase. Thus, the cps1+ product might be a catalytic or an associated copurifying subunit of the fission yeast 1,3-beta-D-glucan synthase that plays an essential role in cell wall synthesis.


This article has been cited by other articles:

  • Sharifmoghadam, M. R., Valdivieso, M.-H. (2009). The Fission Yeast SEL1 Domain Protein Cfh3p: A NOVEL REGULATOR OF THE GLUCAN SYNTHASE Bgs1p WHOSE FUNCTION IS MORE RELEVANT UNDER STRESS CONDITIONS. J. Biol. Chem. 284: 11070-11079 [Abstract] [Full Text]  
  • Ishihara, S., Hirata, A., Nogami, S., Beauvais, A., Latge, J.-P., Ohya, Y. (2007). Homologous Subunits of 1,3-Beta-Glucan Synthase Are Important for Spore Wall Assembly in Saccharomyces cerevisiae. Eukaryot Cell 6: 143-156 [Abstract] [Full Text]  
  • Ha, Y.-s., Covert, S. F., Momany, M. (2006). FsFKS1, the 1,3-{beta}-Glucan Synthase from the Caspofungin-Resistant Fungus Fusarium solani.. Eukaryot Cell 5: 1036-1042 [Abstract] [Full Text]  
  • Morrell-Falvey, J. L., Ren, L., Feoktistova, A., Haese, G. D., Gould, K. L. (2005). Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p. J. Cell Sci. 118: 5563-5573 [Abstract] [Full Text]  
  • Santos, B., Martin-Cuadrado, A. B., Vazquez de Aldana, C. R., del Rey, F., Perez, P. (2005). Rho4 GTPase Is Involved in Secretion of Glucanases during Fission Yeast Cytokinesis. Eukaryot Cell 4: 1639-1645 [Abstract] [Full Text]  
  • Martin-Cuadrado, A. B., Morrell, J. L., Konomi, M., An, H., Petit, C., Osumi, M., Balasubramanian, M., Gould, K. L., del Rey, F., de Aldana, C. R. V. (2005). Role of Septins and the Exocyst Complex in the Function of Hydrolytic Enzymes Responsible for Fission Yeast Cell Separation. Mol. Biol. Cell 16: 4867-4881 [Abstract] [Full Text]  
  • Grun, C. H., Hochstenbach, F., Humbel, B. M., Verkleij, A. J., Sietsma, J. H., Klis, F. M., Kamerling, J. P., Vliegenthart, J. F.G. (2005). The structure of cell wall {alpha}-glucan from fission yeast. Glycobiology 15: 245-257 [Abstract] [Full Text]  
  • Yamashita, A., Sato, M., Fujita, A., Yamamoto, M., Toda, T. (2005). The Roles of Fission Yeast Ase1 in Mitotic Cell Division, Meiotic Nuclear Oscillation, and Cytokinesis Checkpoint Signaling. Mol. Biol. Cell 16: 1378-1395 [Abstract] [Full Text]  
  • Cortes, J. C. G., Carnero, E., Ishiguro, J., Sanchez, Y., Duran, A., Ribas, J. C. (2005). The novel fission yeast (1,3){beta}-D-glucan synthase catalytic subunit Bgs4p is essential during both cytokinesis and polarized growth. J. Cell Sci. 118: 157-174 [Abstract] [Full Text]  
  • Yuasa, T., Hayashi, T., Ikai, N., Katayama, T., Aoki, K., Obara, T., Toyoda, Y., Maruyama, T., Kitagawa, D., Takahashi, K., Nagao, K., Nakaseko, Y., Yanagida, M. (2004). An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation. GENES CELLS 9: 1069-1082 [Abstract] [Full Text]  
  • Cortes, J. C. G., Katoh-Fukui, R., Moto, K., Ribas, J. C., Ishiguro, J. (2004). Schizosaccharomyces pombe Pmr1p Is Essential for Cell Wall Integrity and Is Required for Polarized Cell Growth and Cytokinesis. Eukaryot Cell 3: 1124-1135 [Abstract] [Full Text]  
  • Santos, B., Gutierrez, J., Calonge, T. M., Perez, P. (2003). Novel Rho GTPase Involved in Cytokinesis and Cell Wall Integrity in the Fission Yeast Schizosaccharomyces pombe. Eukaryot Cell 2: 521-533 [Abstract] [Full Text]  
  • Hirayama, S., Sugiura, R., Lu, Y., Maeda, T., Kawagishi, K., Yokoyama, M., Tohda, H., Giga-Hama, Y., Shuntoh, H., Kuno, T. (2003). Zinc Finger Protein Prz1 Regulates Ca2+ but Not Cl- Homeostasis in Fission Yeast. IDENTIFICATION OF DISTINCT BRANCHES OF CALCINEURIN SIGNALING PATHWAY IN FISSION YEAST. J. Biol. Chem. 278: 18078-18084 [Abstract] [Full Text]  
  • Martin-Cuadrado, A. B., Duenas, E., Sipiczki, M., de Aldana, C. R. V., del Rey, F. (2003). The endo-{beta}-1,3-glucanase eng1p is required for dissolution of the primary septum during cell separation in Schizosaccharomyces pombe. J. Cell Sci. 116: 1689-1698 [Abstract] [Full Text]  
  • Martin, V., Garcia, B., Carnero, E., Duran, A., Sanchez, Y. (2003). Bgs3p, a Putative 1,3-{beta}-Glucan Synthase Subunit, Is Required for Cell Wall Assembly in Schizosaccharomyces pombe. Eukaryot Cell 2: 159-169 [Abstract] [Full Text]  
  • Facanha, A. L. O., Appelgren, H., Tabish, M., Okorokov, L., Ekwall, K. (2002). The endoplasmic reticulum cation P-type ATPase Cta4p is required for control of cell shape and microtubule dynamics. JCB 157: 1029-1040 [Abstract] [Full Text]  
  • Liu, J., Tang, X., Wang, H., Oliferenko, S., Balasubramanian, M. K. (2002). The Localization of the Integral Membrane Protein Cps1p to the Cell Division Site is Dependent on the Actomyosin Ring and the Septation-Inducing Network in Schizosaccharomyces pombe. Mol. Biol. Cell 13: 989-1000 [Abstract] [Full Text]  
  • Cortes, J. C. G., Ishiguro, J., Duran, A., Ribas, J. C. (2002). Localization of the (1,3){beta}-D-glucan synthase catalytic subunit homologue Bgs1p/Cps1p from fission yeast suggests that it is involved in septation, polarized growth, mating, spore wall formation and spore germination. J. Cell Sci. 115: 4081-4096 [Abstract] [Full Text]  
  • Feoktistova, A., Magnelli, P., Abeijon, C., Perez, P., Lester, R. L., Dickson, R. C., Gould, K. L. (2001). Coordination Between Fission Yeast Glucan Formation and Growth Requires a Sphingolipase Activity. Genetics 158: 1397-1411 [Abstract] [Full Text]  
  • Beauvais, A., Bruneau, J. M., Mol, P. C., Buitrago, M. J., Legrand, R., Latgé, J. P. (2001). Glucan Synthase Complex of Aspergillus fumigatus. J. Bacteriol. 183: 2273-2279 [Abstract] [Full Text]  
  • Hong, Z., Delauney, A. J., Verma, D. P. S. (2001). A Cell Plate-Specific Callose Synthase and Its Interaction with Phragmoplastin. Plant Cell 13: 755-768 [Abstract] [Full Text]  
  • Calonge, T. M., Nakano, K., Arellano, M., Arai, R., Katayama, S., Toda, T., Mabuchi, I., Perez, P. (2000). Schizosaccharomyces pombe Rho2p GTPase Regulates Cell Wall alpha -Glucan Biosynthesis through the Protein Kinase Pck2p. Mol. Biol. Cell 11: 4393-4401 [Abstract] [Full Text]  
  • Del Poeta, M., Cruz, M. C., Cardenas, M. E., Perfect, J. R., Heitman, J. (2000). Synergistic Antifungal Activities of Bafilomycin A1, Fluconazole, and the Pneumocandin MK-0991/Caspofungin Acetate (L-743,873) with Calcineurin Inhibitors FK506 and L-685,818 against Cryptococcus neoformans. Antimicrob. Agents Chemother. 44: 739-746 [Abstract] [Full Text]  
  • Balasubramanian, M., McCollum, D, Surana, U (2000). Tying the knot: linking cytokinesis to the nuclear cycle. J. Cell Sci. 113: 1503-1513 [Abstract]  
  • Liu, J, Wang, H, Balasubramanian, M. (2000). A checkpoint that monitors cytokinesis in Schizosaccharomyces pombe. J. Cell Sci. 113: 1223-1230 [Abstract]  
  • Liu, J., Wang, H., McCollum, D., Balasubramanian, M. K. (1999). Drc1p/Cps1p, a 1,3-{beta}-Glucan Synthase Subunit, Is Essential for Division Septum Assembly in Schizosaccharomyces pombe. Genetics 153: 1193-1203 [Abstract] [Full Text]  
  • Katayama, S., Hirata, D., Arellano, M., Perez, P., Toda, T. (1999). Fission Yeast alpha -Glucan Synthase Mok1 Requires the Actin Cytoskeleton to Localize the Sites of Growth and Plays an Essential Role in Cell Morphogenesis Downstream of Protein Kinase C Function. JCB 144: 1173-1186 [Abstract] [Full Text]  
  • Thompson, J. R., Douglas, C. M., Li, W., Jue, C. K., Pramanik, B., Yuan, X., Rude, T. H., Toffaletti, D. L., Perfect, J. R., Kurtz, M. (1999). A Glucan Synthase FKS1 Homolog in Cryptococcus neoformans Is Single Copy and Encodes an Essential Function. J. Bacteriol. 181: 444-453 [Abstract] [Full Text]  
  • Arellano, M, Valdivieso, M., Calonge, T., Coll, P., Duran, A, Perez, P (1999). Schizosaccharomyces pombe protein kinase C homologues, pck1p and pck2p, are targets of rho1p and rho2p and differentially regulate cell integrity. J. Cell Sci. 112: 3569-3578 [Abstract]  
  • Fanchiotti, S., Fernandez, F., D'Alessio, C., Parodi, A. J. (1998). The UDP-Glc:Glycoprotein Glucosyltransferase Is Essential for Schizosaccharomyces pombe Viability under Conditions of Extreme Endoplasmic Reticulum Stress. JCB 143: 625-635 [Abstract] [Full Text]  
  • Yada, T., Sugiura, R., Kita, A., Itoh, Y., Lu, Y., Hong, Y., Kinoshita, T., Shuntoh, H., Kuno, T. (2001). Its8, a Fission Yeast Homolog of Mcd4 and Pig-n, Is Involved in GPI Anchor Synthesis and Shares an Essential Function with Calcineurin in Cytokinesis. J. Biol. Chem. 276: 13579-13586 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»