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J. Bacteriol., 01 1998, 143-151, Vol 180, No. 1
Copyright © 1998, American Society for Microbiology

Molecular cloning and physical mapping of the daptomycin gene cluster from Streptomyces roseosporus [In Process Citation]

MA Mchenney, TJ Hosted, BS Dehoff, PR Rosteck Jr and RH Baltz
Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA.

The daptomycin biosynthetic gene cluster of Streptomyces roseosporus was analyzed by Tn5099 mutagenesis, molecular cloning, partial DNA sequencing, and insertional mutagenesis with cloned segments of DNA. The daptomycin biosynthetic gene cluster spans at least 50 kb and is located about 400 to 500 kb from one end of the approximately 7,100-kb linear chromosome. We identified two peptide synthetase coding regions interrupted by a 10- to 20-kb region that may encode other functions in lipopeptide biosynthesis.


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