J. Bacteriol., Jan 1998, 186-189, Vol 180, No. 1
Copyright © 1998, American Society for Microbiology

Ammonification in Bacillus subtilis utilizing dissimilatory nitrite reductase is dependent on resDE [In Process Citation]

T Hoffmann, N Frankenberg, M Marino and D Jahn
Institut fur Organische Chemie und Biochemie, Albert-Ludwigs- Universitat Freiburg, Germany.

During anaerobic nitrate respiration Bacillus subtilis reduces nitrate via nitrite to ammonia. No denitrification products were observed. B. subtilis wild-type cells and a nitrate reductase mutant grew anaerobically with nitrite as an electron acceptor. Oxygen-sensitive dissimilatory nitrite reductase activity was demonstrated in cell extracts prepared from both strains with benzyl viologen as an electron donor and nitrite as an electron acceptor. The anaerobic expression of the discovered nitrite reductase activity was dependent on the regulatory system encoded by resDE. Mutation of the gene encoding the regulatory Fnr had no negative effect on dissimilatory nitrite reductase formation.

This article has been cited by other articles:

• Fuchs, S., Pane-Farre, J., Kohler, C., Hecker, M., Engelmann, S. (2007). Anaerobic Gene Expression in Staphylococcus aureus. J. Bacteriol. 189: 4275-4289 [Abstract] [Full Text]  
• Di Giacomo, M., Paolino, M., Silvestro, D., Vigliotta, G., Imperi, F., Visca, P., Alifano, P., Parente, D. (2007). Microbial Community Structure and Dynamics of Dark Fire-Cured Tobacco Fermentation. Appl. Environ. Microbiol. 73: 825-837 [Abstract] [Full Text]  
• Hartig, E., Hartmann, A., Schatzle, M., Albertini, A. M., Jahn, D. (2006). The Bacillus subtilis nrdEF Genes, Encoding a Class Ib Ribonucleotide Reductase, Are Essential for Aerobic and Anaerobic Growth. Appl. Environ. Microbiol. 72: 5260-5265 [Abstract] [Full Text]  
• Reents, H., Munch, R., Dammeyer, T., Jahn, D., Hartig, E. (2006). The Fnr Regulon of Bacillus subtilis. J. Bacteriol. 188: 1103-1112 [Abstract] [Full Text]  
• Wolfe, A. J. (2005). The Acetate Switch. Microbiol. Mol. Biol. Rev. 69: 12-50 [Abstract] [Full Text]  
• Barbosa, T. M., Serra, C. R., La Ragione, R. M., Woodward, M. J., Henriques, A. O. (2005). Screening for Bacillus Isolates in the Broiler Gastrointestinal Tract. Appl. Environ. Microbiol. 71: 968-978 [Abstract] [Full Text]  
• Hartig, E., Geng, H., Hartmann, A., Hubacek, A., Munch, R., Ye, R. W., Jahn, D., Nakano, M. M. (2004). Bacillus subtilis ResD Induces Expression of the Potential Regulatory Genes yclJK upon Oxygen Limitation. J. Bacteriol. 186: 6477-6484 [Abstract] [Full Text]  
• Marino, M., Ramos, H. C., Hoffmann, T., Glaser, P., Jahn, D. (2001). Modulation of Anaerobic Energy Metabolism of Bacillus subtilis by arfM (ywiD). J. Bacteriol. 183: 6815-6821 [Abstract] [Full Text]  
• Ye, R. W., Tao, W., Bedzyk, L., Young, T., Chen, M., Li, L. (2000). Global Gene Expression Profiles of Bacillus subtilis Grown under Anaerobic Conditions. J. Bacteriol. 182: 4458-4465 [Abstract] [Full Text]  
• Cruz Ramos, H., Hoffmann, T., Marino, M., Nedjari, H., Presecan-Siedel, E., Dreesen, O., Glaser, P., Jahn, D. (2000). Fermentative Metabolism of Bacillus subtilis: Physiology and Regulation of Gene Expression. J. Bacteriol. 182: 3072-3080 [Abstract] [Full Text]  
• Marino, M., Hoffmann, T., Schmid, R., Möbitz, H., Jahn, D. (2000). Changes in protein synthesis during the adaptation of Bacillus subtilis to anaerobic growth conditions. Microbiology 146: 97-105 [Abstract] [Full Text]  
• Azarkina, N., Siletsky, S., Borisov, V., von Wachenfeldt, C., Hederstedt, L., Konstantinov, A. A. (1999). A Cytochrome bb'-type Quinol Oxidase in Bacillus subtilis Strain 168. J. Biol. Chem. 274: 32810-32817 [Abstract] [Full Text]  
• Homuth, G., Rompf, A., Schumann, W., Jahn, D. (1999). Transcriptional Control of Bacillus subtilis hemN and hemZ. J. Bacteriol. 181: 5922-5929 [Abstract] [Full Text]  
• Nakano, M. M., Hoffmann, T., Zhu, Y., Jahn, D. (1998). Nitrogen and Oxygen Regulation of Bacillus subtilis nasDEF Encoding NADH-Dependent Nitrite Reductase by TnrA and ResDE. J. Bacteriol. 180: 5344-5350 [Abstract] [Full Text]