This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, H.-P.
Right arrow Articles by Walker, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, H.-P.
Right arrow Articles by Walker, G. C.

 Previous Article  |  Next Article 

J. Bacteriol., Jan 1998, 20-26, Vol 180, No. 1
Copyright © 1998, American Society for Microbiology

Succinoglycan production by Rhizobium meliloti is regulated through the ExoS-ChvI two-component regulatory system [In Process Citation]

HP Cheng and GC Walker
Biology Department, Massachusetts Institute of Technology, Cambridge 02139, USA.

The Rhizobium meliloti exoS gene is involved in regulating the production of succinoglycan, which plays a crucial role in the establishment of the symbiosis between R. meliloti Rm1021 and its host plant, alfalfa. The exoS96::Tn5 mutation causes the upregulation of the succinoglycan biosynthetic genes, thereby resulting in the overproduction of succinoglycan. Through cloning and sequencing, we found that the exoS gene is a close homolog of the Agrobacterium tumefaciens chvG gene, which has been proposed to encode the sensor protein of the ChvG-ChvI two-component regulatory system, a member of the EnvZ-OmpR family. Further analyses revealed the existence of a newly discovered A. tumefaciens chvI homolog located just upstream of the R. meliloti exoS gene. R. meliloti ChvI may serve as the response regulator of ExoS in a two-component regulatory system. By using ExoS- specific antibodies, it was found that the ExoS protein cofractionated with membrane proteins, suggesting that it is located in the cytoplasmic membrane. By using the same antibodies, it was shown that the exoS96::Tn5 allele encodes an N-terminal truncated derivative of ExoS. The cytoplasmic histidine kinase domain of ExoS was expressed in Escherichia coli and purified, as was the R. meliloti ChvI protein. The ChvI protein autophosphorylated in the presence of acetylphosphate, and the ExoS cytoplasmic domain fragment autophosphorylated at a histidine residue in the presence of ATP. The ChvI protein was phosphorylated in the presence of ATP only when the histidine kinase domain of ExoS was also present. We propose a model for regulation of succinoglycan production by R. meliloti through the ExoS-ChvI two-component regulatory system.


This article has been cited by other articles:

  • Pinedo, C. A., Gage, D. J. (2009). HPrK Regulates Succinate-Mediated Catabolite Repression in the Gram-Negative Symbiont Sinorhizobium meliloti. J. Bacteriol. 191: 298-309 [Abstract] [Full Text]  
  • Hoang, H. H., Gurich, N., Gonzalez, J. E. (2008). Regulation of Motility by the ExpR/Sin Quorum-Sensing System in Sinorhizobium meliloti. J. Bacteriol. 190: 861-871 [Abstract] [Full Text]  
  • Yuan, Z.-C., Liu, P., Saenkham, P., Kerr, K., Nester, E. W. (2008). Transcriptome Profiling and Functional Analysis of Agrobacterium tumefaciens Reveals a General Conserved Response to Acidic Conditions (pH 5.5) and a Complex Acid-Mediated Signaling Involved in Agrobacterium-Plant Interactions. J. Bacteriol. 190: 494-507 [Abstract] [Full Text]  
  • Glenn, S. A., Gurich, N., Feeney, M. A., Gonzalez, J. E. (2007). The ExpR/Sin Quorum-Sensing System Controls Succinoglycan Production in Sinorhizobium meliloti. J. Bacteriol. 189: 7077-7088 [Abstract] [Full Text]  
  • Vriezen, J. A. C., de Bruijn, F. J., Nusslein, K. (2007). Responses of Rhizobia to Desiccation in Relation to Osmotic Stress, Oxygen, and Temperature. Appl. Environ. Microbiol. 73: 3451-3459 [Full Text]  
  • Gibson, K. E., Barnett, M. J., Toman, C. J., Long, S. R., Walker, G. C. (2007). The Symbiosis Regulator CbrA Modulates a Complex Regulatory Network Affecting the Flagellar Apparatus and Cell Envelope Proteins. J. Bacteriol. 189: 3591-3602 [Abstract] [Full Text]  
  • Keating, D. H. (2007). Sinorhizobium meliloti SyrA Mediates the Transcriptional Regulation of Genes Involved in Lipopolysaccharide Sulfation and Exopolysaccharide Biosynthesis. J. Bacteriol. 189: 2510-2520 [Abstract] [Full Text]  
  • Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  
  • Soto, M. J., Sanjuan, J., Olivares, J. (2006). Rhizobia and plant-pathogenic bacteria: common infection weapons.. Microbiology 152: 3167-3174 [Abstract] [Full Text]  
  • Gibson, K. E., Campbell, G. R., Lloret, J., Walker, G. C. (2006). CbrA Is a Stationary-Phase Regulator of Cell Surface Physiology and Legume Symbiosis in Sinorhizobium meliloti.. J. Bacteriol. 188: 4508-4521 [Abstract] [Full Text]  
  • Zhang, X.-S., Cheng, H.-P. (2006). Identification of Sinorhizobium meliloti Early Symbiotic Genes by Use of a Positive Functional Screen. Appl. Environ. Microbiol. 72: 2738-2748 [Abstract] [Full Text]  
  • Liu, P., Wood, D., Nester, E. W. (2005). Phosphoenolpyruvate Carboxykinase Is an Acid-Induced, Chromosomally Encoded Virulence Factor in Agrobacterium tumefaciens. J. Bacteriol. 187: 6039-6045 [Abstract] [Full Text]  
  • Manterola, L., Moriyon, I., Moreno, E., Sola-Landa, A., Weiss, D. S., Koch, M. H. J., Howe, J., Brandenburg, K., Lopez-Goni, I. (2005). The Lipopolysaccharide of Brucella abortus BvrS/BvrR Mutants Contains Lipid A Modifications and Has Higher Affinity for Bactericidal Cationic Peptides. J. Bacteriol. 187: 5631-5639 [Abstract] [Full Text]  
  • Luo, L., Yao, S.-Y., Becker, A., Ruberg, S., Yu, G.-Q., Zhu, J.-B., Cheng, H.-P. (2005). Two New Sinorhizobium meliloti LysR-Type Transcriptional Regulators Required for Nodulation. J. Bacteriol. 187: 4562-4572 [Abstract] [Full Text]  
  • Brencic, A., Winans, S. C. (2005). Detection of and Response to Signals Involved in Host-Microbe Interactions by Plant-Associated Bacteria. Microbiol. Mol. Biol. Rev. 69: 155-194 [Abstract] [Full Text]  
  • Yao, S.-Y., Luo, L., Har, K. J., Becker, A., Ruberg, S., Yu, G.-Q., Zhu, J.-B., Cheng, H.-P. (2004). Sinorhizobium meliloti ExoR and ExoS Proteins Regulate both Succinoglycan and Flagellum Production. J. Bacteriol. 186: 6042-6049 [Abstract] [Full Text]  
  • Roset, M. S., Ciocchini, A. E., Ugalde, R. A., Inon de Iannino, N. (2004). Molecular Cloning and Characterization of cgt, the Brucella abortus Cyclic {beta}-1,2-Glucan Transporter Gene, and Its Role in Virulence. Infect. Immun. 72: 2263-2271 [Abstract] [Full Text]  
  • Tsolis, R. M. (2002). Comparative genome analysis of the alpha -proteobacteria: Relationships between plant and animal pathogens and host specificity. Proc. Natl. Acad. Sci. USA 99: 12503-12505 [Full Text]  
  • Li, L., Jia, Y., Hou, Q., Charles, T. C., Nester, E. W., Pan, S. Q. (2002). A global pH sensor: Agrobacterium sensor protein ChvG regulates acid-inducible genes on its two chromosomes and Ti plasmid. Proc. Natl. Acad. Sci. USA 99: 12369-12374 [Abstract] [Full Text]  
  • Guzman-Verri, C., Manterola, L., Sola-Landa, A., Parra, A., Cloeckaert, A., Garin, J., Gorvel, J.-P., Moriyon, I., Moreno, E., Lopez-Goni, I. (2002). The two-component system BvrR/BvrS essential for Brucellaabortus virulence regulates the expression of outer membrane proteins with counterparts in members of the Rhizobiaceae. Proc. Natl. Acad. Sci. USA 99: 12375-12380 [Abstract] [Full Text]  
  • Briones, G., Inon de Iannino, N., Roset, M., Vigliocco, A., Paulo, P. S., Ugalde, R. A. (2001). Brucella abortus Cyclic {beta}-1,2-Glucan Mutants Have Reduced Virulence in Mice and Are Defective in Intracellular Replication in HeLa Cells. Infect. Immun. 69: 4528-4535 [Abstract] [Full Text]  
  • Peng, W.-T., Banta, L. M., Charles, T. C., Nester, E. W. (2001). The chvH Locus of Agrobacterium Encodes a Homologue of an Elongation Factor Involved in Protein Synthesis. J. Bacteriol. 183: 36-45 [Abstract] [Full Text]  
  • Summers, M. L., Botero, L. M., Busse, S. C., McDermott, T. R. (2000). The Sinorhizobium meliloti Lon Protease Is Involved in Regulating Exopolysaccharide Synthesis and Is Required for Nodulation of Alfalfa. J. Bacteriol. 182: 2551-2558 [Abstract] [Full Text]  
  • Perret, X., Staehelin, C., Broughton, W. J. (2000). Molecular Basis of Symbiotic Promiscuity. Microbiol. Mol. Biol. Rev. 64: 180-201 [Abstract] [Full Text]  
  • Liu, M., González, J. E., Willis, L. B., Walker, G. C. (1998). A Novel Screening Method for Isolating Exopolysaccharide-Deficient Mutants. Appl. Environ. Microbiol. 64: 4600-4602 [Abstract] [Full Text]  
  • Cheng, H.-P., Walker, G. C. (1998). Succinoglycan Is Required for Initiation and Elongation of Infection Threads during Nodulation of Alfalfa by Rhizobium meliloti. J. Bacteriol. 180: 5183-5191 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»