This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bylund, G. O.
Right arrow Articles by Wikström, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bylund, G. O.
Right arrow Articles by Wikström, P. M.

 Previous Article  |  Next Article 

J. Bacteriol., Jan 1998, 73-82, Vol 180, No. 1
Copyright © 1998, American Society for Microbiology

RimM and RbfA are essential for efficient processing of 16S rRNA in Escherichia coli [In Process Citation]

GO Bylund, LC Wipemo, LA Lundberg and PM Wikstrom
Department of Microbiology, Umea University, Sweden.

The trmD operon is located at 56.7 min on the genetic map of the Escherichia coli chromosome and contains the genes for ribosomal protein (r-protein) S16, a 21-kDa protein (RimM, formerly called 21K), the tRNA (m1G37)methyltransferase (TrmD), and r-protein L19, in that order. Previously, we have shown that strains from which the rimM gene has been deleted have a sevenfold-reduced growth rate and a reduced translational efficiency. The slow growth and translational deficiency were found to be partly suppressed by mutations in rpsM, which encodes r-protein S13. Further, the RimM protein was shown to have affinity for free ribosomal 30S subunits but not for 30S subunits in the 70S ribosomes. Here we have isolated several new suppressor mutations, most of which seem to be located close to or within the nusA operon at 68.9 min on the chromosome. For at least one of these mutations, increased expression of the ribosome binding factor RbfA is responsible for the suppression of the slow growth and translational deficiency of a deltarimM mutant. Further, the RimM and RbfA proteins were found to be essential for efficient processing of 16S rRNA.


This article has been cited by other articles:

  • Campbell, T. L., Brown, E. D. (2008). Genetic Interaction Screens with Ordered Overexpression and Deletion Clone Sets Implicate the Escherichia coli GTPase YjeQ in Late Ribosome Biogenesis. J. Bacteriol. 190: 2537-2545 [Abstract] [Full Text]  
  • Jain, C. (2008). The E. coli RhlE RNA helicase regulates the function of related RNA helicases during ribosome assembly. RNA 14: 381-389 [Abstract] [Full Text]  
  • Kaczanowska, M., Ryden-Aulin, M. (2007). Ribosome Biogenesis and the Translation Process in Escherichia coli. Microbiol. Mol. Biol. Rev. 71: 477-494 [Abstract] [Full Text]  
  • Suzuki, S., Tatsuguchi, A., Matsumoto, E., Kawazoe, M., Kaminishi, T., Shirouzu, M., Muto, Y., Takemoto, C., Yokoyama, S. (2007). Structural Characterization of the Ribosome Maturation Protein, RimM. J. Bacteriol. 189: 6397-6406 [Abstract] [Full Text]  
  • Jiang, M., Sullivan, S. M., Walker, A. K., Strahler, J. R., Andrews, P. C., Maddock, J. R. (2007). Identification of Novel Escherichia coli Ribosome-Associated Proteins Using Isobaric Tags and Multidimensional Protein Identification Techniques. J. Bacteriol. 189: 3434-3444 [Abstract] [Full Text]  
  • Barkan, A., Klipcan, L., Ostersetzer, O., Kawamura, T., Asakura, Y., Watkins, K. P. (2007). The CRM domain: An RNA binding module derived from an ancient ribosome-associated protein. RNA 13: 55-64 [Abstract] [Full Text]  
  • Jiang, M., Datta, K., Walker, A., Strahler, J., Bagamasbad, P., Andrews, P. C., Maddock, J. R. (2006). The Escherichia coli GTPase CgtAE Is Involved in Late Steps of Large Ribosome Assembly.. J. Bacteriol. 188: 6757-6770 [Abstract] [Full Text]  
  • Roovers, M., Hale, C., Tricot, C., Terns, M. P., Terns, R. M., Grosjean, H., Droogmans, L. (2006). Formation of the conserved pseudouridine at position 55 in archaeal tRNA. Nucleic Acids Res 34: 4293-4301 [Abstract] [Full Text]  
  • LOVGREN, J. M., BYLUND, G. O., SRIVASTAVA, M. K., LUNDBERG, L.A. C., PERSSON, O. P., WINGSLE, G., WIKSTROM, P. M. (2004). The PRC-barrel domain of the ribosome maturation protein RimM mediates binding to ribosomal protein S19 in the 30S ribosomal subunits. RNA 10: 1798-1812 [Abstract] [Full Text]  
  • Bjork, G. R., Nilsson, K. (2003). 1-Methylguanosine-Deficient tRNA of Salmonella enterica Serovar Typhimurium Affects Thiamine Metabolism. J. Bacteriol. 185: 750-759 [Abstract] [Full Text]  
  • Urbonavicius, J., Durand, J. M. B., Bjork, G. R. (2002). Three Modifications in the D and T Arms of tRNA Influence Translation in Escherichia coli and Expression of Virulence Genes in Shigella flexneri. J. Bacteriol. 184: 5348-5357 [Abstract] [Full Text]  
  • Lovgren, J. M., Wikstrom, P. M. (2001). The rlmB Gene Is Essential for Formation of Gm2251 in 23S rRNA but Not for Ribosome Maturation in Escherichia coli. J. Bacteriol. 183: 6957-6960 [Abstract] [Full Text]  
  • Bylund, G. O., Lovgren, J. M., Wikstrom, P. M. (2001). Characterization of Mutations in the metY-nusA-infB Operon That Suppress the Slow Growth of a {Delta}rimM Mutant. J. Bacteriol. 183: 6095-6106 [Abstract] [Full Text]  
  • Lovgren, J. M., Wikstrom, P. M. (2001). Hybrid Protein between Ribosomal Protein S16 and RimM of Escherichia coli Retains the Ribosome Maturation Function of Both Proteins. J. Bacteriol. 183: 5352-5357 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»