This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fang, L.
Right arrow Articles by Inouye, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fang, L.
Right arrow Articles by Inouye, M.

 Previous Article  |  Next Article 

J. Bacteriol., Jan 1998, 90-95, Vol 180, No. 1
Copyright © 1998, American Society for Microbiology

Role of the cold-box region in the 5' untranslated region of the cspA mRNA in its transient expression at low temperature in Escherichia coli [In Process Citation]

L Fang, Y Hou and M Inouye
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.

Upon temperature downshift, a group of proteins called cold shock proteins, such as CspA, CspB, and CsdA, are transiently induced in Escherichia coli. However, when the 5' untranslated region (5' UTR) of cspA mRNA is overproduced at low temperature, the expression of cold shock genes is prolonged or derepressed. It has been proposed that this effect is due to highly conserved 11-base sequences designated the "cold box" existing in the 5' UTRs of cspA, cspB, and csdA. Here, we demonstrate that the overproduction of the 5' UTR of not only cspA but also cspB and csdA mRNAs causes derepression of all three genes at the same time. Conversely, when the cold-box region was deleted from the cspA 5' UTR its derepression function was abolished. The amount of mRNA from the chromosomal cspA gene was much higher in cells overproducing the wild-type 5' UTR by means of a plasmid than it was in cells overproducing the cold-box-deleted 5' UTR. The stability of the chromosomal cspA mRNA in cells overproducing the wild-type 5' UTR was almost identical to that in cells overproducing the cold-box-deleted 5' UTR. Therefore, the derepression of cspA caused by overproduction of 5' UTR at the end of the acclimation phase occurs at the level of transcription but not by mRNA stabilization, indicating that the cold- box region plays a negative role in cspA transcription in cold shock- adapted cells. The role of the cold-box region was further confirmed with a cspA mutant strain containing a cold-box-deleted cspA gene integrated into the chromosome, which showed a high level of constitutive production of CspA but not CspB during exponential growth at low temperature.


This article has been cited by other articles:

  • Singh, A. K., Pindi, P. K., Dube, S., Sundareswaran, V. R., Shivaji, S. (2009). Importance of trmE for Growth of the Psychrophile Pseudomonas syringae at Low Temperatures. Appl. Environ. Microbiol. 75: 4419-4426 [Abstract] [Full Text]  
  • Zeeb, M., Max, K. E.A., Weininger, U., Low, C., Sticht, H., Balbach, J. (2006). Recognition of T-rich single-stranded DNA by the cold shock protein Bs-CspB in solution. Nucleic Acids Res 34: 4561-4571 [Abstract] [Full Text]  
  • Bresolin, G., Neuhaus, K., Scherer, S., Fuchs, T. M. (2006). Transcriptional Analysis of Long-Term Adaptation of Yersinia enterocolitica to Low-Temperature Growth.. J. Bacteriol. 188: 2945-2958 [Abstract] [Full Text]  
  • Derzelle, S., Hallet, B., Ferain, T., Delcour, J., Hols, P. (2002). Cold Shock Induction of the cspL Gene in Lactobacillus plantarum Involves Transcriptional Regulation. J. Bacteriol. 184: 5518-5523 [Abstract] [Full Text]  
  • Xia, B., Ke, H., Jiang, W., Inouye, M. (2002). The Cold Box Stem-loop Proximal to the 5'-End of the Escherichia coli cspA Gene Stabilizes Its mRNA at Low Temperature. J. Biol. Chem. 277: 6005-6011 [Abstract] [Full Text]  
  • Janiyani, K. L., Ray, M. K. (2002). Cloning, Sequencing, and Expression of the Cold-Inducible hutU Gene from the Antarctic Psychrotrophic Bacterium Pseudomonas syringae. Appl. Environ. Microbiol. 68: 1-10 [Abstract] [Full Text]  
  • Kim, B. H., Bang, I. S., Lee, S. Y., Hong, S. K., Bang, S. H., Lee, I. S., Park, Y. K. (2001). Expression of cspH, Encoding the Cold Shock Protein in Salmonella enterica Serovar Typhimurium UK-1. J. Bacteriol. 183: 5580-5588 [Abstract] [Full Text]  
  • Yamanaka, K., Inouye, M. (2001). Selective mRNA Degradation by Polynucleotide Phosphorylase in Cold Shock Adaptation in Escherichia coli. J. Bacteriol. 183: 2808-2816 [Abstract] [Full Text]  
  • Derzelle, S., Hallet, B., Francis, K. P., Ferain, T., Delcour, J., Hols, P. (2000). Changes in cspL, cspP, and cspC mRNA Abundance as a Function of Cold Shock and Growth Phase in Lactobacillus plantarum. J. Bacteriol. 182: 5105-5113 [Abstract] [Full Text]  
  • Chamot, D., Owttrim, G. W. (2000). Regulation of Cold Shock-Induced RNA Helicase Gene Expression in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 182: 1251-1256 [Abstract] [Full Text]  
  • Lortie, L.-A., Pelletier, M., Vadeboncoeur, C., Frenette, M. (2000). The gene encoding IIABLMan in Streptococcus salivarius is part of a tetracistronic operon encoding a phosphoenolpyruvate:mannose/glucose phosphotransferase system. Microbiology 146: 677-685 [Abstract] [Full Text]  
  • O'Connell, K. P., Thomashow, M. F. (2000). Transcriptional Organization and Regulation of a Polycistronic Cold Shock Operon in Sinorhizobium meliloti RM1021 Encoding Homologs of the Escherichia coli Major Cold Shock Gene cspA and Ribosomal Protein Gene rpsU. Appl. Environ. Microbiol. 66: 392-400 [Abstract] [Full Text]  
  • Lopez, M. M., Yutani, K., Makhatadze, G. I. (1999). Interactions of the Major Cold Shock Protein of Bacillus subtilis CspB with Single-stranded DNA Templates of Different Base Composition. J. Biol. Chem. 274: 33601-33608 [Abstract] [Full Text]  
  • Sahara, T., Suzuki, M., Tsuruha, J.-I., Takada, Y., Fukunaga, N. (1999). cis-Acting Elements Responsible for Low-Temperature-Inducible Expression of the Gene Coding for the Thermolabile Isocitrate Dehydrogenase Isozyme of a Psychrophilic Bacterium, Vibrio sp. Strain ABE-1. J. Bacteriol. 181: 2602-2611 [Abstract] [Full Text]  
  • Wang, N., Yamanaka, K., Inouye, M. (1999). CspI, the Ninth Member of the CspA Family of Escherichia coli, Is Induced upon Cold Shock. J. Bacteriol. 181: 1603-1609 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Uhlmann-Schiffler, H., Rossler, O. G., Stahl, H. (2002). The mRNA of DEAD Box Protein p72 Is Alternatively Translated into an 82-kDa RNA Helicase. J. Biol. Chem. 277: 1066-1075 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»