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J Bacteriol, May 1998, p. 2616-2622, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular and Genetic Analysis of Two Closely Linked Genes That Encode, Respectively, a Protein Phosphatase 1/2A/2B Homolog and a Protein Kinase Homolog in the Cyanobacterium Anabaena sp. Strain PCC 7120

Cheng-Cai Zhang,1,* Aline Friry,1,dagger and Ling Peng2

Unité d'Immunotechnologie et Microbiologie Moléculaires, Ecole Supérieure de Biotechnologie de Strasbourg, Université Louis Pasteur de Strasbourg, F-67400 Illkirch,1 and Laboratoire de Chimie Bio-organique, CNRS-UMR 7514, Faculté de Pharmacie, Université Louis Pasteur de Strasbourg, F-67401 Illkirch Cedex,2 France

Received 26 December 1997/Accepted 11 March 1998

Reversible protein phosphorylation plays important roles in signal transduction. One gene, prpA, encoding a protein similar to eukaryotic types of phosphoprotein phosphatases PP1, PP2A, and PP2B, was cloned from the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. Interestingly, a eukaryotic-type protein kinase gene, pknE, was found 301 bp downstream of prpA. This unusual genetic arrangement provides the opportunity for study about how the balance between protein phosphorylation and dephosphorylation can regulate cellular activities. Both proteins were overproduced in Escherichia coli and used to raise polyclonal antibodies. Immunodetection and RNA/DNA hybridization experiments suggest that these two genes are unlikely to be coexpressed, despite their close genetic linkage. PrpA is expressed constitutively under different nitrogen conditions, while PknE expression varies according to the nature of the nitrogen source. Inactivation analysis in vivo suggests that PrpA and PknE function to ensure a correct level of phosphorylation of the targets in order to regulate similar biological processes such as heterocyst structure formation and nitrogen fixation.

* Corresponding author. Mailing address: Unité d'Immunotechnologie et Microbiologie Moléculaires, ESBS, Boulevard Sébastien Brandt, F-67400 Illkirch, France. Phone: (33) 3 8865 5290. Fax: (33) 3 8865 5330. E-mail: cczhang{at}esbs1.u-strasbg.fr.

dagger Present address: LAB, 92270 Bois-Colombes, France.

J Bacteriol, May 1998, p. 2616-2622, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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