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J Bacteriol, May 1998, p. 2630-2635, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Periplasmic Cyclodextrin Binding Protein CymE from Klebsiella oxytoca and Its Role in Maltodextrin and Cyclodextrin Transport

Markus Pajatsch,1 Maria Gerhart,1 Ralf Peist,2 Reinhold Horlacher,2 Winfried Boos,2 and August Böck1,*

Lehrstuhl für Mikrobiologie der Universität München, D-80638 München,1 and Lehrstuhl für Mikrobiologie der Universität Konstanz, D-78434 Konstanz,2 Germany

Received 6 November 1997/Accepted 9 March 1998

Klebsiella oxytoca M5a1 has the capacity to transport and to metabolize alpha -, beta - and gamma -cyclodextrins. Cyclodextrin transport is mediated by the products of the cymE, cymF, cymG, cymD, and cymA genes, which are functionally homologous to the malE, malF, malG, malK, and lamB gene products of Escherichia coli. CymE, which is the periplasmic binding protein, has been overproduced and purified. By substrate-induced fluorescence quenching, the binding of ligands was analyzed. CymE bound alpha -cyclodextrin, beta -cyclodextrin, and gamma -cyclodextrin, with dissociation constants (Kd) of 0.02, 0.14 and 0.30 µM, respectively, and linear maltoheptaose, with a Kd of 70 µM. In transport experiments, alpha -cyclodextrin was taken up by the cym system of K. oxytoca three to five times less efficiently than maltohexaose by the E. coli maltose system. Besides alpha -cyclodextrin, maltohexaose was also taken up by the K. oxytoca cym system, but because of the inability of maltodextrins to induce the cym system, growth of E. coli mal mutants on linear maltodextrin was not observed when the cells harbored only the cym uptake system. Strains which gained this capacity by mutation could easily be selected, however.

* Corresponding author. Mailing address: Institute of Genetics and Microbiology, Maria-Ward-Straße 1a, D-80638 München, Germany. Phone: 89-17919856. Fax: 89-17919862. E-mail: august.boeck{at}rz.uni-muenchen.de.

J Bacteriol, May 1998, p. 2630-2635, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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