This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mongkolsuk, S.
Right arrow Articles by Chamnongpol, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mongkolsuk, S.
Right arrow Articles by Chamnongpol, S.

 Previous Article  |  Next Article 

J Bacteriol, May 1998, p. 2636-2643, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification and Characterization of a New Organic Hydroperoxide Resistance (ohr) Gene with a Novel Pattern of Oxidative Stress Regulation from Xanthomonas campestris pv. phaseoli

Skorn Mongkolsuk,1,2,* Wipa Praituan,1,2 Suvit Loprasert,1 Mayuree Fuangthong,1 and Sangpen Chamnongpol2,dagger

Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210,1 and Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400,2 Thailand

Received 12 December 1997/Accepted 9 March 1998

We have isolated a new organic hydroperoxide resistance (ohr) gene from Xanthomonas campestris pv. phaseoli. This was done by complementation of an Escherichia coli alkyl hydroperoxide reductase mutant with an organic hydroperoxide-hypersensitive phenotype. ohr encodes a 14.5-kDa protein. Its amino acid sequence shows high homology with several proteins of unknown function. An ohr mutant was subsequently constructed, and it showed increased sensitivity to both growth-inhibitory and killing concentrations of organic hydroperoxides but not to either H2O2 or superoxide generators. No alterations in sensitivity to other oxidants or stresses were observed in the mutant. ohr had interesting expression patterns in response to low concentrations of oxidants. It was highly induced by organic hydroperoxides, weakly induced by H2O2, and not induced at all by a superoxide generator. The novel regulation pattern of ohr suggests the existence of a second organic hydroperoxide-inducible system that differs from the global peroxide regulator system, OxyR. Expression of ohr in various bacteria tested conferred increased resistance to tert-butyl hydroperoxide killing, but this was not so for wild-type Xanthomonas strains. The organic hydroperoxide hypersensitivity of ohr mutants could be fully complemented by expression of ohr or a combination of ahpC and ahpF and could be partially complemented by expression ahpC alone. The data suggested that Ohr was a new type of organic hydroperoxide detoxification protein.

* Corresponding author. Mailing address: Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210, Thailand. Phone: (662) 574-0622. Fax: (662) 574-2027. E-mail: scsmk{at}mucc.mahidol.ac.th.

dagger Present address: Laboratory of Genetics, Gent University, Gent B-9000, Belgium.

J Bacteriol, May 1998, p. 2636-2643, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Saikolappan, S., Sasindran, S. J., Yu, H. D., Baseman, J. B., Dhandayuthapani, S. (2009). The Mycoplasma genitalium MG_454 Gene Product Resists Killing by Organic Hydroperoxides. J. Bacteriol. 191: 6675-6682 [Abstract] [Full Text]  
  • Jenkins, C., Samudrala, R., Geary, S. J., Djordjevic, S. P. (2008). Structural and Functional Characterization of an Organic Hydroperoxide Resistance Protein from Mycoplasma gallisepticum. J. Bacteriol. 190: 2206-2216 [Abstract] [Full Text]  
  • Soonsanga, S., Fuangthong, M., Helmann, J. D. (2007). Mutational Analysis of Active Site Residues Essential for Sensing of Organic Hydroperoxides by Bacillus subtilis OhrR. J. Bacteriol. 189: 7069-7076 [Abstract] [Full Text]  
  • Oh, S.-Y., Shin, J.-H., Roe, J.-H. (2007). Dual Role of OhrR as a Repressor and an Activator in Response to Organic Hydroperoxides in Streptomyces coelicolor. J. Bacteriol. 189: 6284-6292 [Abstract] [Full Text]  
  • Harrison, A., Ray, W. C., Baker, B. D., Armbruster, D. W., Bakaletz, L. O., Munson, R. S. Jr. (2007). The OxyR Regulon in Nontypeable Haemophilus influenzae. J. Bacteriol. 189: 1004-1012 [Abstract] [Full Text]  
  • Chuchue, T., Tanboon, W., Prapagdee, B., Dubbs, J. M., Vattanaviboon, P., Mongkolsuk, S. (2006). ohrR and ohr Are the Primary Sensor/Regulator and Protective Genes against Organic Hydroperoxide Stress in Agrobacterium tumefaciens. J. Bacteriol. 188: 842-851 [Abstract] [Full Text]  
  • Allenby, N. E. E., O'Connor, N., Pragai, Z., Ward, A. C., Wipat, A., Harwood, C. R. (2005). Genome-Wide Transcriptional Analysis of the Phosphate Starvation Stimulon of Bacillus subtilis. J. Bacteriol. 187: 8063-8080 [Abstract] [Full Text]  
  • Vattanaviboon, P., Seeanukun, C., Whangsuk, W., Utamapongchai, S., Mongkolsuk, S. (2005). Important Role for Methionine Sulfoxide Reductase in the Oxidative Stress Response of Xanthomonas campestris pv. phaseoli. J. Bacteriol. 187: 5831-5836 [Abstract] [Full Text]  
  • Klomsiri, C., Panmanee, W., Dharmsthiti, S., Vattanaviboon, P., Mongkolsuk, S. (2005). Novel Roles of ohrR-ohr in Xanthomonas Sensing, Metabolism, and Physiological Adaptive Response to Lipid Hydroperoxide. J. Bacteriol. 187: 3277-3281 [Abstract] [Full Text]  
  • Hoper, D., Volker, U., Hecker, M. (2005). Comprehensive Characterization of the Contribution of Individual SigB-Dependent General Stress Genes to Stress Resistance of Bacillus subtilis. J. Bacteriol. 187: 2810-2826 [Abstract] [Full Text]  
  • Banjerdkij, P., Vattanaviboon, P., Mongkolsuk, S. (2005). Exposure to Cadmium Elevates Expression of Genes in the OxyR and OhrR Regulons and Induces Cross-Resistance to Peroxide Killing Treatment in Xanthomonas campestris. Appl. Environ. Microbiol. 71: 1843-1849 [Abstract] [Full Text]  
  • Chauvatcharin, N., Atichartpongkul, S., Utamapongchai, S., Whangsuk, W., Vattanaviboon, P., Mongkolsuk, S. (2005). Genetic and physiological analysis of the major OxyR-regulated katA from Xanthomonas campestris pv. phaseoli. Microbiology 151: 597-605 [Abstract] [Full Text]  
  • Marceau, A., Zagorec, M., Chaillou, S., Mera, T., Champomier-Verges, M.-C. (2004). Evidence for Involvement of at Least Six Proteins in Adaptation of Lactobacillus sakei to Cold Temperatures and Addition of NaCl. Appl. Environ. Microbiol. 70: 7260-7268 [Abstract] [Full Text]  
  • Meunier-Jamin, C., Kapp, U., Leonard, G. A., McSweeney, S. (2004). The Structure of the Organic Hydroperoxide Resistance Protein from Deinococcus radiodurans: DO CONFORMATIONAL CHANGES FACILITATE RECYCLING OF THE REDOX DISULFIDE?. J. Biol. Chem. 279: 25830-25837 [Abstract] [Full Text]  
  • Sturny, R., Cam, K., Gutierrez, C., Conter, A. (2003). NhaR and RcsB Independently Regulate the osmCp1 Promoter of Escherichia coli at Overlapping Regulatory Sites. J. Bacteriol. 185: 4298-4304 [Abstract] [Full Text]  
  • Cussiol, J. R. R., Alves, S. V., Antonio de Oliveira, M., Netto, L. E. S. (2003). Organic Hydroperoxide Resistance Gene Encodes a Thiol-dependent Peroxidase. J. Biol. Chem. 278: 11570-11578 [Abstract] [Full Text]  
  • Baker, L. M. S., Poole, L. B. (2003). Catalytic Mechanism of Thiol Peroxidase from Escherichia coli. SULFENIC ACID FORMATION AND OVEROXIDATION OF ESSENTIAL CYS61. J. Biol. Chem. 278: 9203-9211 [Abstract] [Full Text]  
  • Vattanaviboon, P., Whangsuk, W., Mongkolsuk, S. (2003). A Suppressor of the Menadione-Hypersensitive Phenotype of a Xanthomonas campestris pv. phaseoli oxyR Mutant Reveals a Novel Mechanism of Toxicity and the Protective Role of Alkyl Hydroperoxide Reductase. J. Bacteriol. 185: 1734-1738 [Abstract] [Full Text]  
  • Tellez-Sosa, J., Soberon, N., Vega-Segura, A., Torres-Marquez, M. E., Cevallos, M. A. (2002). The Rhizobium etli cyaC Product: Characterization of a Novel Adenylate Cyclase Class. J. Bacteriol. 184: 3560-3568 [Abstract] [Full Text]  
  • Fuangthong, M., Helmann, J. D. (2002). The OhrR repressor senses organic hydroperoxides by reversible formation of a cysteine-sulfenic acid derivative. Proc. Natl. Acad. Sci. USA 99: 6690-6695 [Abstract] [Full Text]  
  • Shea, R. J., Mulks, M. H. (2002). ohr, Encoding an Organic Hydroperoxide Reductase, Is an In Vivo-Induced Gene in Actinobacillus pleuropneumoniae. Infect. Immun. 70: 794-802 [Abstract] [Full Text]  
  • Eskra, L., Canavessi, A., Carey, M., Splitter, G. (2001). Brucella abortus Genes Identified following Constitutive Growth and Macrophage Infection. Infect. Immun. 69: 7736-7742 [Abstract] [Full Text]  
  • Toesca, I., Perard, C., Bouvier, J., Gutierrez, C., Conter, A. (2001). The transcriptional activator NhaR is responsible for the osmotic induction of osmCp1, a promoter of the stress-inducible gene osmC in Escherichia coli. Microbiology 147: 2795-2803 [Abstract] [Full Text]  
  • Sukchawalit, R., Loprasert, S., Atichartpongkul, S., Mongkolsuk, S. (2001). Complex Regulation of the Organic Hydroperoxide Resistance Gene (ohr) from Xanthomonas Involves OhrR, a Novel Organic Peroxide-Inducible Negative Regulator, and Posttranscriptional Modifications. J. Bacteriol. 183: 4405-4412 [Abstract] [Full Text]  
  • Fuangthong, M., Atichartpongkul, S., Mongkolsuk, S., Helmann, J. D. (2001). OhrR Is a Repressor of ohrA, a Key Organic Hydroperoxide Resistance Determinant in Bacillus subtilis. J. Bacteriol. 183: 4134-4141 [Abstract] [Full Text]  
  • Atichartpongkul, S., Loprasert, S., Vattanaviboon, P., Whangsuk, W., Helmann, J. D., Mongkolsuk, S. (2001). Bacterial Ohr and OsmC paralogues define two protein families with distinct functions and patterns of expression. Microbiology 147: 1775-1782 [Abstract] [Full Text]  
  • Rincé, A., Giard, J.-C., Pichereau, V., Flahaut, S., Auffray, Y. (2001). Identification and Characterization of gsp65, an Organic Hydroperoxide Resistance (ohr) Gene Encoding a General Stress Protein in Enterococcus faecalis. J. Bacteriol. 183: 1482-1488 [Abstract] [Full Text]  
  • Vattanaviboon, P., Sriprang, R., Mongkolsuk, S. (2001). Catalase has a novel protective role against electrophile killing of Xanthomonas. Microbiology 147: 491-498 [Abstract] [Full Text]  
  • Ochsner, U. A., Hassett, D. J., Vasil, M. L. (2001). Genetic and Physiological Characterization of ohr, Encoding a Protein Involved in Organic Hydroperoxide Resistance in Pseudomonas aeruginosa. J. Bacteriol. 183: 773-778 [Abstract] [Full Text]  
  • Mongkolsuk, S., Whangsuk, W., Vattanaviboon, P., Loprasert, S., Fuangthong, M. (2000). A Xanthomonas Alkyl Hydroperoxide Reductase Subunit C (ahpC) Mutant Showed an Altered Peroxide Stress Response and Complex Regulation of the Compensatory Response of Peroxide Detoxification Enzymes. J. Bacteriol. 182: 6845-6849 [Abstract] [Full Text]  
  • Sriprang, R., Vattanaviboon, P., Mongkolsuk, S. (2000). Exposure of Phytopathogenic Xanthomonas spp. to Lethal Concentrations of Multiple Oxidants Affects Bacterial Survival in a Complex Manner. Appl. Environ. Microbiol. 66: 4017-4021 [Abstract] [Full Text]  
  • Yamamoto, Y., Higuchi, M., Poole, L. B., Kamio, Y. (2000). Role of the dpr Product in Oxygen Tolerance in Streptococcus mutans. J. Bacteriol. 182: 3740-3747 [Abstract] [Full Text]  
  • Higuchi, M., Yamamoto, Y., Poole, L. B., Shimada, M., Sato, Y., Takahashi, N., Kamio, Y. (1999). Functions of Two Types of NADH Oxidases in Energy Metabolism and Oxidative Stress of Streptococcus mutans. J. Bacteriol. 181: 5940-5947 [Abstract] [Full Text]  
  • Völker, U., Andersen, K. K., Antelmann, H., Devine, K. M., Hecker, M. (1998). One of Two OsmC Homologs in Bacillus subtilis Is Part of the sigma B-Dependent General Stress Regulon. J. Bacteriol. 180: 4212-4218 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»