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J Bacteriol, May 1998, p. 2744-2748, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

An rRNA Fragment and Its Antisense Can Alter Decoding of Genetic Information

Alexey L. Arkov,1 Alexander Mankin,2 and Emanuel J. Murgola1,*

Department of Molecular Genetics, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030,1 and Center for Pharmaceutical Biotechnology, The University of Illinois, Chicago, Illinois 606072

Received 2 February 1998/Accepted 18 March 1998

rRNA plays a central role in protein synthesis and is intimately involved in the initiation, elongation, and termination stages of translation. However, the mode of its participation in these reactions, particularly as to the decoding of genetic information, remains elusive. In this paper, we describe a new approach that allowed us to identify an rRNA segment whose function is likely to be related to translation termination. By screening an expression library of random rRNA fragments, we identified a fragment of the Escherichia coli 23S rRNA (nucleotides 74 to 136) whose expression caused readthrough of UGA nonsense mutations in certain codon contexts in vivo. The antisense RNA fragment produced a similar effect, but in neither case was readthrough of UAA or UAG observed. Since termination at UGA in E. coli specifically requires release factor 2 (RF2), our data suggest that the fragments interfere with RF2-dependent termination.

* Corresponding author. Mailing address: Molecular Genetics (Box 11), M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030. Phone: (713) 792-8939. Fax: (713) 794-4295. E-mail: ejm{at}mdacc.tmc.edu.

J Bacteriol, May 1998, p. 2744-2748, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.