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J Bacteriol, May 1998, p. 2779-2781, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Oligoribonuclease Is Encoded by a Highly Conserved Gene in the 3'-5' Exonuclease Superfamily

Xiaolan Zhang,dagger Liuqin Zhu,Dagger and Murray P. Deutscher*

Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida 33101-6129

Received 18 December 1997/Accepted 6 March 1998

Oligoribonuclease, a 3'-to-5' exoribonuclease specific for small oligoribonucleotides, was purified to homogeneity from extracts of Escherichia coli. The purified protein is an alpha 2 dimer of 40 kDa. NH2-terminal sequence analysis of the protein identified the gene encoding oligoribonuclease as yjeR (o204a), a previously reported open reading frame located at 94 min on the E. coli chromosome. However, as a consequence of the sequence information, the translation start site of this open reading frame has been revised. Cloning of yjeR led to overexpression of oligoribonuclease activity, and interruption of the cloned gene with a kanamycin resistance cassette eliminated the overexpression. On the basis of these data, we propose that yjeR be renamed orn. Orthologs of oligoribonuclease are present in a wide range of organisms, extending up to humans.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, P.O. Box 016129, Miami, FL 33101-6129. Phone: (305) 243-3150. Fax: (305) 243-3955. E-mail: mdeutsch{at}mednet.med.miami.edu.

dagger Present address: Department of Neuroscience, Tufts University Medical School, Boston, MA.

Dagger Present address: Institute of Biophysics, Academia Sinica, Beijing, China.

J Bacteriol, May 1998, p. 2779-2781, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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