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J Bacteriol, May 1998, p. 2792-2795, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mutational Analysis of the Phosphate-Binding Loop of Rhizobium meliloti DctD, a sigma 54-Dependent Activator

Yan Gao, Ying-Kai Wang, and Timothy R. Hoover*

Department of Microbiology, University of Georgia, Athens, Georgia 30602

Received 23 December 1997/Accepted 19 March 1998

The phosphate-binding loop of sigma 54-dependent activators is thought to participate in ATP binding and/or hydrolysis. Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif in Rhizobium meliloti DctD disrupted transcriptional activation and ATP hydrolysis. Interestingly, substitution of alanine at position 7 also affected DNA binding.

* Corresponding author. Mailing address: Department of Microbiology, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602. Phone: (706) 542-2675. Fax: (706) 542-2674. E-mail: hoover{at}bscr.uga.edu.

J Bacteriol, May 1998, p. 2792-2795, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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