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J Bacteriol, May 1998, p. 2792-2795, Vol. 180, No. 10
Department of Microbiology, University of
Georgia, Athens, Georgia 30602
Received 23 December 1997/Accepted 19 March 1998
The phosphate-binding loop of
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Mutational Analysis of the Phosphate-Binding Loop
of Rhizobium meliloti DctD, a
54-Dependent Activator
54-dependent
activators is thought to participate in ATP binding and/or hydrolysis.
Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif in
Rhizobium meliloti DctD disrupted transcriptional
activation and ATP hydrolysis. Interestingly, substitution of alanine
at position 7 also affected DNA binding.
*
Corresponding author. Mailing address: Department of
Microbiology, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602. Phone: (706) 542-2675. Fax: (706) 542-2674. E-mail:
hoover{at}bscr.uga.edu.
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