This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Redenbach, M.
Right arrow Articles by Kinashi, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Redenbach, M.
Right arrow Articles by Kinashi, H.

 Previous Article

J Bacteriol, May 1998, p. 2796-2799, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning and Physical Mapping of the EcoRI Fragments of the Giant Linear Plasmid SCP1

Matthias Redenbach,1,2 Kazuya Ikeda,1 Masayuki Yamasaki,1 and Haruyasu Kinashi1,*

Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima 739-8527, Japan,1 and Genome Research Unit, Department of Genetics, Kaiserslautern University, D-67653 Kaiserslautern, Germany2

Received 25 November 1997/Accepted 10 March 1998

A cosmid library was constructed for the 350-kb giant linear plasmid SCP1 and aligned on a successive linear map. Only a 0.8-kb gap has remained uncloned in the terminal inverted repeats close to both ends. Partial digestion of the aligned cosmids with EcoRI and hybridization with the flanking fragments of the vector enabled physical mapping of all of the EcoRI fragments. On this map, the methylenomycin biosynthetic gene cluster, the insertion sequence IS466, and the sapCDE genes coding for spore-associated proteins were localized.

* Corresponding author. Mailing address: Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima 739-8527, Japan. Phone: 81-824-7869. Fax: 81-824-24-7869. E-mail: kinashi{at}ipc.hiroshima-u.ac.jp.

J Bacteriol, May 1998, p. 2796-2799, Vol. 180, No. 10
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Arakawa, K., Mochizuki, S., Yamada, K., Noma, T., Kinashi, H. (2007). {gamma}-Butyrolactone autoregulator-receptor system involved in lankacidin and lankamycin production and morphological differentiation in Streptomyces rochei. Microbiology 153: 1817-1827 [Abstract] [Full Text]  
  • Ramos, J. L., Martinez-Bueno, M., Molina-Henares, A. J., Teran, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., Tobes, R. (2005). The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69: 326-356 [Abstract] [Full Text]  
  • Yamasaki, M., Kinashi, H. (2004). Two Chimeric Chromosomes of Streptomyces coelicolor A3(2) Generated by Single Crossover of the Wild-Type Chromosome and Linear Plasmid SCP1. J. Bacteriol. 186: 6553-6559 [Abstract] [Full Text]  
  • Shi, L., Zhang, W. (2004). Comparative analysis of eukaryotic-type protein phosphatases in two streptomycete genomes. Microbiology 150: 2247-2256 [Abstract] [Full Text]  
  • Stecker, C., Johann, A., Herzberg, C., Averhoff, B., Gottschalk, G. (2003). Complete Nucleotide Sequence and Genetic Organization of the 210-Kilobase Linear Plasmid of Rhodococcus erythropolis BD2. J. Bacteriol. 185: 5269-5274 [Abstract] [Full Text]  
  • Yamasaki, M., Ikuto, Y., Ohira, A., Chater, K., Kinashi, H. (2003). Limited regions of homology between linear and circular plasmids encoding methylenomycin biosynthesis in two independently isolated streptomycetes. Microbiology 149: 1351-1356 [Abstract] [Full Text]  
  • Gust, B., Spatz, K., Spychaj, A., Redenbach, M. (2001). Region-Specific Transcriptional Activity in the Genome of Streptomyces coelicolor A3(2). Appl. Environ. Microbiol. 67: 3598-3602 [Abstract] [Full Text]  
  • Wang, Z.-X., Li, S.-M., Heide, L. (2000). Identification of the Coumermycin A1 Biosynthetic Gene Cluster of Streptomyces rishiriensis DSM 40489. Antimicrob. Agents Chemother. 44: 3040-3048 [Abstract] [Full Text]  
  • Yamasaki, M., Miyashita, K., Cullum, J., Kinashi, H. (2000). A Complex Insertion Sequence Cluster at a Point of Interaction between the Linear Plasmid SCP1 and the Linear Chromosome of Streptomyces coelicolor A3(2). J. Bacteriol. 182: 3104-3110 [Abstract] [Full Text]  
  • Steffensky, M., Mühlenweg, A., Wang, Z.-X., Li, S.-M., Heide, L. (2000). Identification of the Novobiocin Biosynthetic Gene Cluster of Streptomyces spheroides NCIB 11891. Antimicrob. Agents Chemother. 44: 1214-1222 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»