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J Bacteriol, June 1998, p. 2895-2900, Vol. 180, No. 11
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Nonnative Proteins Induce Expression of the Bacillus subtilis CIRCE Regulon

Axel Mogk,1 Andrea Völker,2 Susanne Engelmann,3 Michael Hecker,3 Wolfgang Schumann,1 and Uwe Völker2,*

Institut für Genetik, Universität Bayreuth, 95440 Bayreuth,1 Laboratorium für Mikrobiologie, Philipps-Universität, and MPI für terrestrische Mikrobiologie, 35043 Marburg,2 and Institut für Mikrobiologie and Molekularbiologie and Ernst-Moritz-Arndt-Universität, 17487 Greifswald,3 Germany

Received 23 December 1997/Accepted 24 March 1998

The chaperone-encoding groESL and dnaK operons constitute the CIRCE regulon of Bacillus subtilis. Both operons are under negative control of the repressor protein HrcA, which interacts with the CIRCE operator and whose activity is modulated by the GroESL chaperone machine. In this report, we demonstrate that induction of the CIRCE regulon can also be accomplished by ethanol stress and puromycin. Introduction of the hrcA gene and a transcriptional fusion under the control of the CIRCE operator into Escherichia coli allowed induction of this fusion by heat shock, ethanol stress, and overproduction of GroESL substrates. The expression level of this hrcA-bgaB fusion inversely correlated with the amount of GroE machinery present in the cells. Therefore, all inducing conditions seem to lead to induction via titration of the GroE chaperonins by the increased level of nonnative proteins formed. Puromycin treatment failed to induce the sigma B-dependent general stress regulon, indicating that nonnative proteins in general do not trigger this response. Reconstitution of HrcA-dependent heat shock regulation of B. subtilis in E. coli and complementation of E. coli groESL mutants by B. subtilis groESL indicate that the GroE chaperonin systems of the two bacterial species are functionally exchangeable.

* Corresponding author. Mailing address: Philipps-Universität, Laboratorium für Mikrobiologie, Karl-von-Frisch-Strasse, 35043 Marburg, Germany. Phone: 49 6421 283478. Fax: 49 6421 288979. E-mail: voelker{at}su1701.biologie.uni-marburg.de.

J Bacteriol, June 1998, p. 2895-2900, Vol. 180, No. 11
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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