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J Bacteriol, June 1998, p. 2992-2994, Vol. 180, No. 11
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Determining the Optimal Thymidine Concentration for Growing Thyminus Escherichia coli Strains

Felipe Molina, Alfonso Jiménez-Sánchez, and Elena C. Guzmán*

Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, E-06080 Badajoz, Spain

Received 3 December 1997/Accepted 1 April 1998

Changes of thymidine concentration in the growth medium affect the chromosome replication time of Thy- strains without at the same time causing a detectable difference in the growth rate (R. H. Pritchard and A. Zaritsky, Nature 226:126-131, 1970). Consequently, the optimal thymidine concentration cannot be determined by ascertaining which concentration produces the highest growth rate. Here we present a method for determining the optimal thymidine concentration of any Thy- Escherichia coli strain. Using this method, we found that the E. coli "wild-type" strain MG1655 has a partial Thy- phenotype.

* Corresponding author. Mailing address: Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, E-06080 Badajoz, Spain. Phone: 34-24-274800, ext. 9050. Fax: 34-24-274657 or -271304. E-mail: eguzman{at}unex.es.

J Bacteriol, June 1998, p. 2992-2994, Vol. 180, No. 11
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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