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J Bacteriol, June 1998, p. 3013-3016, Vol. 180, No. 11
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Analysis of cis-Acting Elements Required for bfpA Expression in Enteropathogenic Escherichia coli

Victor H. Bustamante, Edmundo Calva, and Jose Luis Puente*

Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62250, Mexico

Received 3 December 1997/Accepted 25 March 1998

bfpA expression in enteropathogenic Escherichia coli is regulated by growth medium, temperature, and ammonium concentration and requires the BfpT protein (also called PerA), a member of the AraC family of transcriptional activators. Site-directed and PCR random mutagenesis, as well as deletion analysis of the bfpA upstream regulatory region, supported assignment of the promoter elements and demonstrated that the cis-acting elements that mediate BfpT-dependent regulation of bfpA are located between positions -85 and -46. Interestingly, this region shares 73% identity with a 40-bp-long AT-rich tract located upstream of the bfpT gene, which is essential for bfpT autoregulation.

* Corresponding author. Mailing address: Instituto de Biotecnología, UNAM, Apdo. Postal 510-3, Cuernavaca, Morelos 62250, Mexico. Phone: (52) (73) 29-1621. Fax: (52) (73) 13-8673. E-mail: puente{at}ibt.unam.mx.

J Bacteriol, June 1998, p. 3013-3016, Vol. 180, No. 11
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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