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J Bacteriol, June 1998, p. 3137-3143, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Anaerobic Growth, a Property Horizontally Transferred by an Hfr-Like Mechanism among Extreme Thermophiles

Sandra Ramírez-Arcos, Luis A. Fernández-Herrero, Irma Marín, and José Berenguer*

Centro de Biología Molecular "Severo Ochoa," UAM-CSIC, Universidad Autónoma de Madrid, 28049 Madrid, Spain

Received 16 December 1997/Accepted 29 March 1998

Despite the fact that the extreme thermophilic bacteria belonging to the genus Thermus are classified as strict aerobes, we have shown that Thermus thermophilus HB8 (ATCC 27634) can grow anaerobically when nitrate is present in the growth medium. This strain-specific property is encoded by a respiratory nitrate reductase gene cluster (nar) whose expression is induced by anoxia and nitrate (S. Ramírez-Arcos, L. A. Fernández-Herrero, and J. Berenguer, Biochim. Biophys. Acta, 1396:215-1997). We show here that this nar operon can be transferred by conjugation to an aerobic Thermus strain, enabling it to grow under anaerobic conditions. We show that this transfer takes place through a DNase-insensitive mechanism which, as for the Hfr (high frequency of recombination) derivatives of Escherichia coli, can also mobilize other chromosomal markers in a time-dependent way. Three lines of evidence are presented to support a genetic linkage between nar and a conjugative plasmid integrated into the chromosome. First, the nar operon is absent from a plasmid-free derivative and from a closely related strain. Second, we have identified an origin for autonomous replication (oriV) overlapping the last gene of the nar cluster. Finally, the mating time required for the transfer of the nar operon is in good agreement with the time expected if the transfer origin (oriT) were located nearby and downstream of nar.

* Corresponding author. Mailing address: Centro de Biología Molecular "Severo Ochoa," UAM-CSIC, Universidad Autónoma de Madrid, 28049 Madrid, Spain. Phone: 34-91-3978099. Fax: 34-91-3978087. E-mail: JBERENGUER{at}trasto.cbm.uam.es.

J Bacteriol, June 1998, p. 3137-3143, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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