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J Bacteriol, June 1998, p. 3174-3180, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Multiple Transcriptional Control of the Lactococcus lactis trp Operon

Raul Raya,dagger Jacek Bardowski,Dagger Paal S. Andersen,§ S. Dusko Ehrlich, and Alain Chopin*

Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France

Received 2 December 1997/Accepted 23 March 1998

The Lactococcus lactis trpEGDCFBA operon is preceded by a noncoding leader region. Transcriptional studies of the trp operon revealed three transcripts with respective sizes of 8 kb (encompassing the entire operon), 290 bases, and 160 bases (corresponding to parts of the leader region). These transcripts most likely result from initiation at the unique Ptrp promoter, transcription termination at either T1 (upstream of the trp operon) or T2 (downstream of the trp operon), and/or processing. Three parameters were shown to differentially affect the amount of these transcripts: (i) following tryptophan depletion, the amount of the 8-kb transcript increases 300- to 500-fold; (ii) depletion in any amino acid increased transcription initiation about fourfold; and (iii) upon entry into stationary phase the amount of the 8-kb transcript decreases abruptly. The tryptophan-dependent transcription control is exerted through transcription antitermination.

* Corresponding author. Mailing address: INRA, Laboratoire de Génétique Microbienne, 78352 Jouy-en-Josas, France. Phone: (33) 1 34 65 25 30. Fax: (33) 1 34 65 25 21. E-mail: achopin{at}biotec.jouy.inra.fr.

dagger Present address: CERELA, Chacabuco 145, 4000 S.M. de Tucuman, Tucuman, Argentina.

Dagger Present address: IBB-PAN, Zaklad Biochemii Drobnoustrojow, Ul. Pawinskiego 5A, 02-106 Warszawa, Poland.

§ Present address: Biotechnological Institute, 2800 Lyngby, Denmark.

J Bacteriol, June 1998, p. 3174-3180, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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