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J Bacteriol, June 1998, p. 3205-3208, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Effects of Carbon Source on Expression of Fo Genes
and on the Stoichiometry of the c Subunit in the
F1Fo ATPase of Escherichia
coli
Randy A.
Schemidt,
Jun
Qu,
James R.
Williams, and
William S. A.
Brusilow*
Department of Biochemistry and Molecular
Biology, Wayne State University School of Medicine, Detroit,
Michigan 48201
Received 18 February 1998/Accepted 14 April 1998
Expression of the genes for the membrane-bound Fo
sector of the Escherichia coli F1Fo
proton-translocating ATPase can respond to changes in metabolic
conditions, and these changes are reflected in alterations in the
subunit stoichiometry of the oligomeric Fo proton channel.
Transcriptional and translational lacZ fusions to the
promoter and to two Fo genes show that, during growth on the nonfermentable carbon source succinate, transcription of the operon
and translation of uncB, encoding the a subunit of
Fo, are higher than during growth on glucose. In contrast,
translation of the uncE gene, encoding the c subunit of
Fo, is higher during growth on glucose than during growth
on succinate. Translation rates of both uncB and
uncE change as culture density increases, but transcription
rates do not. Quantitation of the c stoichiometry shows that more c
subunits are assembled into the F1Fo ATPase in
cells grown on glucose than in cells grown on succinate. E. coli therefore appears to have a mechanism for regulating the composition and, presumably, the function of the ATPase in response to
metabolic circumstances.
*
Corresponding author. Mailing address: Department of
Biochemistry and Molecular Biology, Wayne State University School of Medicine, Scott Hall, 540 E. Canfield Ave., Detroit, MI 48201. Phone:
(313) 577-6659. Fax: (313) 577-2765. E-mail:
wbrusilo{at}med.wayne.edu.
J Bacteriol, June 1998, p. 3205-3208, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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