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J Bacteriol, June 1998, p. 3205-3208, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effects of Carbon Source on Expression of Fo Genes and on the Stoichiometry of the c Subunit in the F1Fo ATPase of Escherichia coli

Randy A. Schemidt, Jun Qu, James R. Williams, and William S. A. Brusilow*

Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, Michigan 48201

Received 18 February 1998/Accepted 14 April 1998

Expression of the genes for the membrane-bound Fo sector of the Escherichia coli F1Fo proton-translocating ATPase can respond to changes in metabolic conditions, and these changes are reflected in alterations in the subunit stoichiometry of the oligomeric Fo proton channel. Transcriptional and translational lacZ fusions to the promoter and to two Fo genes show that, during growth on the nonfermentable carbon source succinate, transcription of the operon and translation of uncB, encoding the a subunit of Fo, are higher than during growth on glucose. In contrast, translation of the uncE gene, encoding the c subunit of Fo, is higher during growth on glucose than during growth on succinate. Translation rates of both uncB and uncE change as culture density increases, but transcription rates do not. Quantitation of the c stoichiometry shows that more c subunits are assembled into the F1Fo ATPase in cells grown on glucose than in cells grown on succinate. E. coli therefore appears to have a mechanism for regulating the composition and, presumably, the function of the ATPase in response to metabolic circumstances.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Scott Hall, 540 E. Canfield Ave., Detroit, MI 48201. Phone: (313) 577-6659. Fax: (313) 577-2765. E-mail: wbrusilo{at}med.wayne.edu.

J Bacteriol, June 1998, p. 3205-3208, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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