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J Bacteriol, June 1998, p. 3222-3226, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The Glucose Kinase of Bacillus subtilis

Pierre Skarlatos and Michael K. Dahl*

Lehrstuhl für Mikrobiologie, Institut für Mikrobiologie, Biochemie und Genetik, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany

Received 23 February 1998/Accepted 6 April 1998

The open reading frame yqgR (now termed glcK), which had been sequenced as part of the genome project, encodes a glucose kinase of Bacillus subtilis. A 1.1-kb DNA fragment containing glcK complemented an Escherichia coli strain deficient in glucose kinase activity. Insertional mutagenesis of glcK resulted in a complete inactivation of glucose kinase activity in crude protein extracts, indicating that B. subtilis contains one major glucose kinase. The glcK gene encodes a 321-residue protein with a molecular mass of 33.5 kDa. The glucose kinase was overexpressed as a fusion protein to a six-His affinity tag and purified to homogeneity. The enzyme had Km values for ATP and glucose of 0.77 and 0.24 mM, respectively, and a Vmax of 93 µmol min-1 mg-1. A B. subtilis strain deficient for glucose kinase grew at the same rate on different carbon sources tested, including disaccharides such as maltose, trehalose, and sucrose.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, FAU Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. Phone: 49-9131-858094. Fax: 49-9131-858082. E-mail: mdahl{at}biologie.uni-erlangen.de.

J Bacteriol, June 1998, p. 3222-3226, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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