This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, I-P.
Right arrow Articles by Michel, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, I-P.
Right arrow Articles by Michel, H.

 Previous Article  |  Next Article 

J Bacteriol, June 1998, p. 3227-3232, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning, Sequencing, and Characterization of the recA Gene from Rhodopseudomonas viridis and Construction of a recA Strain

I-Peng Chen and Hartmut Michel*

Max-Planck-Institut für Biophysik, Frankfurt am Main, D-60528, Germany

Received 15 September 1997/Accepted 7 April 1998

A recombination-deficient strain of the phototrophic bacterium Rhodopseudomonas viridis was constructed for the homologous expression of modified photosynthetic reaction center genes. The R. viridis recA gene was cloned and subsequently deleted from the R. viridis genome. The cloned R. viridis recA gene shows high identity to known recA genes and was able to complement the Rec- phenotype of a Rhizobium meliloti recA strain. The constructed R. viridis recA strain showed the general Rec- phenotype, i.e., increased sensitivity to DNA damage and severely impaired recombination ability. The latter property of this strain will be of advantage in particular for expression of modified, nonfunctional photosynthetic reaction centers which are not as yet available.

* Corresponding author. Mailing address: Max-Planck-Institut für Biophysik, Heinrich-Hoffmann-Str. 7, D-60528 Frankfurt am Main, Germany. Phone: (49) 69 96769401. Fax: (49) 69 96769423. E-mail: baumh{at}mpibp-frankfurt.mpg.de.

J Bacteriol, June 1998, p. 3227-3232, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Roux, C. M., Booth, N. J., Bellaire, B. H., Gee, J. M., Roop, R. M. II, Kovach, M. E., Tsolis, R. M., Elzer, P. H., Ennis, D. G. (2006). RecA and RadA Proteins of Brucella abortus Do Not Perform Overlapping Protective DNA Repair Functions following Oxidative Burst.. J. Bacteriol. 188: 5187-5195 [Abstract] [Full Text]  
  • Douka, E., Koukkou, A. I., Vartholomatos, G., Frillingos, S., Papamichael, E. M., Drainas, C. (1999). A Zymomonas mobilis Mutant with Delayed Growth on High Glucose Concentrations. J. Bacteriol. 181: 4598-4604 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»