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J Bacteriol, June 1998, p. 3227-3232, Vol. 180, No. 12
Max-Planck-Institut für Biophysik,
Frankfurt am Main, D-60528, Germany
Received 15 September 1997/Accepted 7 April 1998
A recombination-deficient strain of the phototrophic bacterium
Rhodopseudomonas viridis was constructed for the homologous expression of modified photosynthetic reaction center genes. The R. viridis recA gene was cloned and subsequently
deleted from the R. viridis genome. The cloned
R. viridis recA gene shows high identity to known
recA genes and was able to complement the Rec
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Cloning, Sequencing, and Characterization of the recA
Gene from Rhodopseudomonas viridis and Construction of a
recA Strain
phenotype of a Rhizobium meliloti recA strain. The
constructed R. viridis recA strain showed the general
Rec
phenotype, i.e., increased sensitivity to DNA damage
and severely impaired recombination ability. The latter property of
this strain will be of advantage in particular for expression of
modified, nonfunctional photosynthetic reaction centers which are not
as yet available.
*
Corresponding author. Mailing address:
Max-Planck-Institut für Biophysik, Heinrich-Hoffmann-Str. 7, D-60528 Frankfurt am Main, Germany. Phone: (49) 69 96769401. Fax: (49)
69 96769423. E-mail: baumh{at}mpibp-frankfurt.mpg.de.
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