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J Bacteriol, June 1998, p. 3245-3249, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Appropriate Expression of Filamentous Phage f1 DNA Replication Genes II and X Requires RNase E-Dependent Processing and Separate mRNAs

Robert J. Kokoskadagger and Deborah A. Steege*

Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710

Received 30 December 1997/Accepted 15 April 1998

The products of in-frame overlapping genes II and X carried by the filamentous phage f1 genome are proteins with required but opposing functions in phage DNA replication. Their normal relative levels are important for continuous production of phage DNA without killing infected Escherichia coli hosts. Here we identify several factors responsible for determining the relative levels of pII and pX and that, if perturbed, alter the normal distribution of phage DNA species in infected hosts. Translation of the two proteins is essentially relegated to separate mRNAs. The mRNAs encoding genes II and X are also differentially sensitive to cleavage dependent on rne, the gene encoding the only E. coli endo-RNase known to have a global role in mRNA stability. Whereas pII levels are limited at the level of mRNA stability, normal pX levels require transcription in sufficient amounts from the promoter for the smaller mRNA encoding only pX.

* Corresponding author. Mailing address: Department of Biochemistry, Box 3711, Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-4098. Fax: (919) 684-5040. E-mail: steege{at}biochem.duke.edu.

dagger Present address: Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280.

J Bacteriol, June 1998, p. 3245-3249, Vol. 180, No. 12
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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