Identification and Characterization of Two Quiescent Porin Genes, nmpC and ompN, in Escherichia coli BE

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Vol. 180, Issue 13, 3388-3392, July 1, 1998

Identification and Characterization of Two Quiescent Porin Genes, nmpC and ompN, in Escherichia coli B^E

Alexej Prilipov, Prashant S. Phale, Ralf Koebnik, Christine Widmer, and Jurg P. Rosenbusch

Department of Microbiology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland

The genomic DNA of the B^E strain of Escherichia coli has been scrutinized to detect porin genes that have not been identifiedso far. Southern blot analysis yielded two DNA segments whichproved highly homologous to, yet distinct from, the ompC, ompF,and phoE porin genes. The two genes were cloned and sequenced.One of them, designated ompN, encodes a porin which, due to lowlevels of expression, has eluded prior identification. The functionalproperties (single-channel conductance) of the OmpN porin, purifiedto homogeneity, closely resemble those of the OmpC porin fromE. coli K-12. The second DNA fragment detected corresponds tothe nmpC gene, which, due to an insertion of an IS1 element inits coding region, is not expressed in E. coli B^E.

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