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Vol. 180, Issue 13, 3400-3404, July 1, 1998

Expression of the bglH Gene of Lactobacillus plantarum Is Controlled by Carbon Catabolite Repression

Rosangela Marasco1, Lidia Muscariello2, Mario Varcamonti3, Maurilio De Felice3, and Margherita Sacco2

1 Dipartimento di Chimica, Università degli Studi di Salerno, 84084 Fisciano (Sa),1 2 Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, 80125 Naples,2 and 3 Sezione di Microbiologia, Dipartimento di Fisiologia Generale ed Ambientale, Università Federico II, 80134 Naples,3 Italy

A newly identified bglH gene coding for a phospho-beta -glucosidase of Lactobacillus plantarum was isolated and expressed in Escherichia coli. The sequence analysis of the cloned DNA fragment showed an open reading frame encoding a 480-amino-acid protein with a calculated molecular mass of 53 kDa. The bglH gene was shown to be expressed on a monocistronic transcriptional unit. Its transcription was repressed 10-fold in L. plantarum cells grown on glucose compared to the beta -glucoside salicin as a sole carbon source. A catabolite-responsive element (CRE) spanning from -3 to +11 with respect to the transcriptional start point was found, and its functionality was assessed by mutational analysis. In vitro and in vivo DNA binding experiments suggested the occurrence of a DNA-protein complex at the CRE site, which would mediate glucose repression of bglH expression.

Copyright © 1998 by American Society for Microbiology


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