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Vol. 180, Issue 13, 3470-3473, July 1, 1998

NOTE
tetA(L) Mutants of a Tetracycline-Sensitive Strain of Bacillus subtilis with the Polynucleotide Phosphorylase Gene Deleted

David H. Bechhofer and Stan J. Stasinopoulos

Department of Biochemistry, Mount Sinai School of Medicine of the City University of New York, New York, New York 10029

A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant mutants. A point mutation in the tetA(L) promoter and a spontaneously occurring tetA(L) gene copy number mutant were characterized.

Copyright © 1998 by American Society for Microbiology


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