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J Bacteriol, July 1998, p. 3529-3532, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Regulation of Biosynthesis of Individual Cellulases in Thermomonospora fusca

Nikolay A. Spiridonovdagger and David B. Wilson*

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853

Received 30 December 1997/Accepted 11 May 1998

Regulation of the biosynthesis of the six cellulases comprising the cellulolytic system of the thermophilic soil bacterium Thermomonospora fusca ER1 was studied. The levels of the individual enzymes produced on different noninducing and inducing carbon sources were determined. The lowest level of cellulase synthesis (3 nM) was observed with xylose as a carbon source, and the highest level (247 to 1,670 nM for different enzymes) was found in cultures grown on microcrystalline cellulose. Endocellulases and exocellulases showed distinctly different regulation patterns. Differences in the regulation of individual enzymes appear to be determined by the specific structural organization of the upstream regulatory sequences of their genes.

* Corresponding author. Mailing address: 458 Biotechnology Building, Cornell University, Ithaca, NY 14853. Phone: (607) 255-5706. Fax: (607) 255-2428. E-mail: dbw3{at}cornell.edu.

dagger Permanent address: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia.

J Bacteriol, July 1998, p. 3529-3532, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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