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J Bacteriol, July 1998, p. 3629-3634, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

RpoS (Sigma-S) Controls Expression of rsmA, a Global Regulator of Secondary Metabolites, Harpin, and Extracellular Proteins in Erwinia carotovoradagger

Asita Mukherjee,1 Yaya Cui,1 Weilei Ma,1 Yang Liu,1 Akira Ishihama,2 Abraham Eisenstark,3 and Arun K. Chatterjee1,*

Department of Plant Pathology, University of Missouri, Columbia, Missouri 652111; Cancer Research Center, Columbia, Missouri 652013; and Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan2

Received 22 January 1998/Accepted 18 May 1998

RpoS (sigma-S or sigma-38) controls a large array of genes that are expressed during stationary phase and under various stress conditions in Escherichia coli and other bacteria. We document here that plant pathogenic and epiphytic Erwinia species, such as E. amylovora; E. carotovora subsp. atroseptica, betavasculorum, and carotovora; E. chrysanthemi; E. herbicola; E. rhapontici; and E. stewartii, possess rpoS genes and produce the alternate sigma factor. We show that rpoS transcription in E. carotovora subsp. carotovora is driven from a major promoter which resides within the nlpD gene located upstream of rpoS as in E. coli. RpoS- E. carotovora subsp. carotovoa strain AC5061, constructed by marker exchange, is more sensitive to hydrogen peroxide, carbon starvation, and acidic pH than its RpoS+ parent strain, AC5006. The basal levels of extracellular pectate lyase, polygalacturonase, and cellulase as well as those of transcripts of E. carotovora subsp. carotovora hrpN (hrpNEcc), the gene for the elicitor of the hypersensitive reaction, are higher in the RpoS- strain than in the RpoS+ parent. Likewise, compared to AC5006, AC5061 causes more extensive maceration of celery petioles. Our findings with the RpoS- mutant and strains carrying multiple copies rpoS+ DNA reveal that rpoS positively controls rsmA expression. We also present evidence that supports the hypothesis that the RpoS effect on extracellular enzyme levels, hrpNEcc expression, and virulence manifests itself by the modulation of rsmA expression.

* Corresponding author. Mailing address: Department of Plant Pathology, College of Agriculture, Food and Natural Resources, University of Missouri-Columbia, 108 Waters Hall, Columbia, Mo. 65211. Phone: (573) 882-2643. Fax: (573) 882-0588. E-mail: achatterjee{at}psu.missouri.edu.

dagger Journal series 12,671 of the Missouri Agricultural Experiment Station.

J Bacteriol, July 1998, p. 3629-3634, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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