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J Bacteriol, July 1998, p. 3719-3723, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Diversity of Cytochrome bc Complexes: Example of the Rieske Protein in Green Sulfur Bacteria

Myriam Brugna,1 Delphine Albouy,2 and Wolfgang Nitschke1,*

Laboratoire de Bioénergétique et Ingénierie des Protéines (UPR 9036), Institut de Biologie Structurale et Microbiologie, 13402 Marseille Cedex 20,1 and Département de Biochimie et de Génétique Moléculaire, Unité de Physiologie Microbienne, Institut Pasteur, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1129, 75724 Paris Cedex 15,2 France

Received 16 March 1998/Accepted 17 May 1998

The Rieske 2Fe2S cluster of Chlorobium limicola forma thiosulfatophilum strain tassajara was studied by electron paramagnetic resonance spectroscopy. Two distinct orientations of its g tensor were observed in oriented samples corresponding to differing conformations of the protein. Only one of the two conformations persisted after treatment with 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. A redox midpoint potential (Em) of +160 mV in the pH range of 6 to 7.7 and a decreasing Em (-60 to -80 mV/pH unit) above pH 7.7 were found. The implications of the existence of differing conformational states of the Rieske protein, as well as of the shape of its Em-versus-pH curve, in green sulfur bacteria are discussed.

* Corresponding author. Mailing address: Laboratoire de Bioénergétique et Ingénierie des Protéines (UPR 9036), Institut de Biologie Structurale et Microbiologie, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Phone: (33) 4 91164435. Fax: (33) 4 91164578. E-mail: nitschke{at}ibsm.cnrs-mrs.fr.

J Bacteriol, July 1998, p. 3719-3723, Vol. 180, No. 14
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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