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Journal of Bacteriology, August 1998, p. 3816-3822, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Physical and Genetic Map of the Obligate Intracellular Bacterium Coxiella burnetii

H. Willems,* Cornelie Jäger, and Georg Baljer

Institute of Hygiene and Infectious Diseases of Animals, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany

Received 10 January 1998/Accepted 3 May 1998

Pulsed-field gel electrophoresis and PCR techniques have been used to construct a NotI macrorestriction map of the obligate intracellular bacterium Coxiella burnetii Nine Mile. The size of the chromosome has been determined to be 2,103 kb comprising 29 NotI restriction fragments. The average resolution is 72.5 kb, or about 3.5% of the genome. Experimental data support the presence of a linear chromosome. Published genes were localized on the physical map by Southern hybridization. One gene, recognized as transposable element, was found to be present in at least nine sites evenly distributed over the whole chromosome. There is only one copy of a 16S rRNA gene. The putative oriC has been located on a 27.5-kb NotI fragment. Gene organization upstream the oriC is almost identical to that of Pseudomonas putida and Bacillus subtilis, whereas gene organization downstream the oriC seems to be unique among bacteria. The physical map will be helpful in investigations of the great heterogeneity in restriction fragment length polymorphism patterns of different isolates and the great variation in genome size. The genetic map will help to determine whether gene order in different isolates is conserved.

* Corresponding author. Mailing address: Institute of Hygiene and Infectious Diseases of Animals, Justus-Liebig-Universität Giessen, Frankfurter Str. 89, D-35392 Giessen, Germany. Phone: (49) 641 99 38308. Fax: (49) 641 99 38309. E-mail: Hermann.Willems{at}vetmed.uni-giessen.de.

Journal of Bacteriology, August 1998, p. 3816-3822, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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