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Journal of Bacteriology, August 1998, p. 3864-3872, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Control by Nutrients of Growth and Cell Cycle Progression in Budding Yeast, Analyzed by Double-Tag Flow Cytometry

Lilia Alberghina,* Carla Smeraldi, Bianca Maria Ranzi, and Danilo Porro

Dipartimento di Fisiologia e Biochimica Generali, Sezione Biochimica Comparata, Università degli Studi di Milano, 20133 Milano, Italy

Received 27 March 1998/Accepted 28 May 1998

To gain insight on the interrelationships of the cellular environment, the properties of growth, and cell cycle progression, we analyzed the dynamic reactions of individual Saccharomyces cerevisiae cells to changes and manipulations of their surroundings. We used a new flow cytometric approach which allows, in asynchronous growing S. cerevisiae populations, tagging of both the cell age and the cell protein content of cells belonging to the different cell cycle set points. Since the cell protein content is a good estimation of the cell size, it is possible to follow the kinetics of the cell size increase during cell cycle progression. The analysis of the findings obtained indicates that both during a nutritional shift-up (from ethanol to glucose) and following the addition of cyclic AMP (cAMP), two important delays are induced. The preexisting cells that at the moment of the nutritional shift-up were cycling before the Start phase delay their entrance into S phase, while cells that were cycling after Start are delayed in their exit from the cycle. The combined effects of the two delays allow the cellular population that preexisted the shift-up to quickly adjust to the new growth condition. The effects of a nutritional shift-down were also determined.

* Corresponding author. Mailing address: Dipartimento di Fisiologia e Biochimica Generali, Sezione Biochimica Comparata, University of Milan, Via Celoria 26, 20133 Milan, Italy. Phone: 39 2 70644801. Fax: 39 2 70632811. E-mail: l.alberg{at}imiucca.csi.unimi.it.

Journal of Bacteriology, August 1998, p. 3864-3872, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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