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Journal of Bacteriology, August 1998, p. 3933-3939, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
The Conjugal Intermediate of Plasmid RSF1010
Inhibits Agrobacterium tumefaciens Virulence and
VirB-Dependent Export of VirE2
Lisa E.
Stahl,
Amy
Jacobs, and
Andrew N.
Binns*
Plant Science Institute, Department of
Biology, University of Pennsylvania, Philadelphia, Pennsylvania
Received 13 February 1998/Accepted 30 May 1998
Agrobacterium tumefaciens causes crown gall disease by
transferring oncogenic, single-stranded DNA (T strand), covalently attached to the VirD2 protein, across the bacterial envelope into plant
cells where its expression results in tumor formation. The single-stranded DNA binding protein VirE2 is also transferred into the
plant cell, though the location at which VirE2 interacts with the T
strand is still under investigation. The movement of the transferred
DNA and VirE2 from A. tumefaciens to the plant cell depends
on the membrane-localized VirB and VirD4 proteins. Further, the
movement of the IncQ broad-host-range plasmid RSF1010 between
Agrobacterium strains or from Agrobacterium to
plants also requires the virB-encoded transfer system. Our
earlier studies showed that the presence of the RSF1010 plasmid in
wild-type strains of Agrobacterium inhibits both their
virulence and their capacity to transport VirE2, as assayed by
coinfection with virE mutants. Here we demonstrate that the
capacity to form a conjugal intermediate of RSF1010 is necessary for
this inhibition, suggesting that the transferred form of the
plasmid competes with the VirD2-T strand and/or VirE2 for a common
export site.
*
Corresponding author. Mailing address: Leidy Labs,
Department of Biology, University of Pennsylvania, Philadelphia, PA
19104-6018. Phone: (215) 898-8684. Fax: (215) 898-8780. E-mail:
abinns{at}sas.upenn.edu.
Journal of Bacteriology, August 1998, p. 3933-3939, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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