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Journal of Bacteriology, August 1998, p. 3967-3972, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Isolation, Characterization, and Expression of the Gene Encoding the beta  Subunit of the Mitochondrial Processing Peptidase from Blastocladiella emersonii

Cíntia Renata Costa Rocha and Suely Lopes Gomes*

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, São Paulo 05599-970, Brazil

Received 12 February 1998/Accepted 22 May 1998

A 2.3-kb BamHI-KpnI fragment was isolated from a partial genomic library and shown by nucleotide sequence analysis to contain the entire coding region of the gene encoding the beta  subunit of the Blastocladiella mitochondrial processing peptidase (beta -MPP). The predicted beta -MPP protein has 465 amino acids and a calculated molecular mass of 50.8 kDa. S1 nuclease protection assays revealed an intron, 209 bp in size, interrupting the coding region between the putative signal sequence and the mature protein. Northern blot analysis showed that beta -MPP mRNA levels decrease significantly during B. emersonii sporulation, reaching basal levels in the zoospore stage. The amount of beta -MPP protein, determined in Western blots, unlike its mRNA, does not vary significantly throughout the fungal life cycle.

* Corresponding author. Mailing address: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Caixa Postal 26.077, São Paulo, SP 05599-970, Brazil. Phone: 55-11-818-3826. Fax: 55-11-815-5579. E-mail: sulgomes{at}quim.iq.usp.br.

Journal of Bacteriology, August 1998, p. 3967-3972, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Simão, R. d. C. G., Gomes, S. L. (2001). Structure, Expression, and Functional Analysis of the Gene Coding for Calmodulin in the Chytridiomycete Blastocladiella emersonii. J. Bacteriol. 183: 2280-2288 [Abstract] [Full Text]  
  • Rocha, C. R. C., Gomes, S. L. (1999). Characterization and Submitochondrial Localization of the alpha Subunit of the Mitochondrial Processing Peptidase from the Aquatic Fungus Blastocladiella emersonii. J. Bacteriol. 181: 4257-4265 [Abstract] [Full Text]  


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