Journal of Bacteriology, August 1998, p. 3978-3982, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Departamento de Biología Molecular,
Received 6 February 1998/Accepted 22 May 1998
In this work, the genes that encode the FliM and FliN proteins of
Rhodobacter sphaeroides were characterized. These genes are part of a large flagellar gene cluster in which six additional open
reading frames encoding products homologous to FliL, FliO, FliP, FliQ,
FliR, and FlhB proteins from other bacteria were identified. The
inactivation of the fliM gene gave a nonflagellate
phenotype (Fla
), suggesting that FliM is required for
flagellar assembly. Complementation analysis of this fliM
mutant indicated that fliM and fliN
transcription starts beyond the 5' end of fliK and
terminates after fliN.
*
Corresponding author. Mailing address: Departamento de
Biología Molecular, Instituto de Investigaciones
Biomédicas UNAM, Apdo Postal 70-228, 04510 México, D. F., Mexico. Phone: (525) 622 38 24. Fax: (525) 622 38 91. E-mail:
rosal{at}servidor.unam.mx.
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