This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Engelberg, D.
Right arrow Articles by Fink, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Engelberg, D.
Right arrow Articles by Fink, G. R.

 Previous Article  |  Next Article 

Journal of Bacteriology, August 1998, p. 3992-3996, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Multicellular Stalk-Like Structures in Saccharomyces cerevisiae

David Engelberg,1,* Avishai Mimran,1 Horacio Martinetto,2 Joel Otto,2 Giora Simchen,3 Michael Karin,2 and Gerald R. Fink4

Department of Biological Chemistry1 and Department of Genetics,3 The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel; Department of Pharmacology, University of California San Diego School of Medicine, La Jolla, California 92093-06362; and Whitehead Institute for Biomedical Research, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142-14794

Received 23 March 1998/Accepted 5 May 1998

Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproducibly in large numbers. Yeast mutants, mutated for control of cell polarity, developmental processes, UV response, and signal transduction cascades were tested and found capable of forming stalk-like structures. We suggest a model that explains the mechanism of stalk formation by mechanical environmental forces. We show that other microorganisms (Candida albicans, Schizosaccharomyces pombe, and Escherichia coli) also form stalks, suggesting that the ability to produce stalks may be a general property of microorganisms. Diploid yeast stalks sporulate at an elevated frequency, raising the possibility that the physiological role of stalks might be disseminating spores.

* Corresponding author. Mailing address: Department of Biological Chemistry, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. Phone: 972 2 6584718. Fax: 972 2 6586448. E-mail: ENGELBER{at}vms.huji.ac.il.

Journal of Bacteriology, August 1998, p. 3992-3996, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Breitkreutz, A., Boucher, L., Breitkreutz, B.-J., Sultan, M., Jurisica, I., Tyers, M. (2003). Phenotypic and Transcriptional Plasticity Directed by a Yeast Mitogen-Activated Protein Kinase Network. Genetics 165: 997-1015 [Abstract] [Full Text]  
  • Scherz, R., Shinder, V., Engelberg, D. (2001). Anatomical Analysis of Saccharomyces cerevisiae Stalk-Like Structures Reveals Spatial Organization and Cell Specialization. J. Bacteriol. 183: 5402-5413 [Abstract] [Full Text]  
  • Stanhill, A., Schick, N., Engelberg, D. (1999). The Yeast Ras/Cyclic AMP Pathway Induces Invasive Growth by Suppressing the Cellular Stress Response. Mol. Cell. Biol. 19: 7529-7538 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»