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Journal of Bacteriology, August 1998, p. 4011-4014, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Functional Expression in Lactobacillus plantarum of xylP Encoding the Isoprimeverose Transporter of Lactobacillus pentosus

Stéphane Chaillou,1,2 Pieter W. Postma,1 and Peter H. Pouwels1,2,*

EC Slater Institute, BioCentrum, University of Amsterdam, 1018 TV Amsterdam,1 and Department of Molecular Genetics and Gene Technology, TNO Nutrition and Food Research Institute, 3700 AJ Zeist,2 The Netherlands

Received 16 March 1998/Accepted 1 June 1998

The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in Lactobacillus plantarum 80, a strain which lacks active isoprimeverose and D-xylose transport activities, it was shown that xylP encodes a transporter. Functional expression of the XylP transporter was shown by uptake of isoprimeverose in L. plantarum 80 cells, and this transport was driven by the proton motive force generated by malolactic fermentation. XylP was unable to catalyze transport of D-xylose.

* Corresponding author. Mailing address: Department of Molecular Genetics and Gene Technology, TNO Nutrition and Food Research Institute, P.O. Box 360, 3700 AJ Zeist, The Netherlands. Phone: 31 30 6944 462. Fax: 31 30 6944 466. E-mail: Pouwels{at}voeding.tno.nl.

Journal of Bacteriology, August 1998, p. 4011-4014, Vol. 180, No. 15
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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