Previous Article | Next Article 
Journal of Bacteriology, August 1998, p. 4233-4242, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Regulation of hepA of
Anabaena sp. Strain PCC 7120 by Elements 5' from the Gene
and by hepK
Jinsong
Zhu,
Renqiu
Kong,
and
C. Peter
Wolk*
MSU-DOE Plant Research Laboratory, Michigan
State University, East Lansing, Michigan 48824
Received 27 March 1998/Accepted 4 June 1998
In Anabaena spp., synthesis of the heterocyst envelope
polysaccharide, required if the cell is to fix dinitrogen under aerobic conditions, is dependent on the gene hepA. A
transcriptional start site of hepA was localized 104 bp 5'
from its translational initiation codon. A 765-bp open reading frame,
denoted hepC, was found farther upstream. Inactivation of
hepC led to constitutive expression of hepA and
prevented the synthesis of heterocyst envelope polysaccharide. However,
the glycolipid layer of the heterocyst envelope was synthesized. A
hepK mutation blocked both the synthesis of the heterocyst
envelope polysaccharide and induction of hepA. The
predicted product of hepK resembles a sensory
protein-histidine kinase of a two-component regulatory system. Analysis
of the region between hepC and hepA indicated
that DNA sequences required for the induction of hepA upon
nitrogen deprivation are present between bp
574 and
440 and between
bp
340 and
169 relative to the transcriptional start site of
hepA. Gel mobility shift assays provided evidence that one
or more proteins bind specifically to the latter sequence. The Fox box
sequence downstream from hepA appeared inessential for the
induction of hepA.
*
Corresponding author. Mailing address: MSU-DOE Plant
Research Laboratory, Michigan State University, East Lansing, MI 48824. Phone: (517) 353-2049. Fax: (517) 353-9168. E-mail:
wolk{at}pilot.msu.edu.

Present address: Department of Entomology, Michigan State
University, East Lansing, MI 48824.

Present address: Department of Microbiology, Michigan State
University, East Lansing, MI 48824.
Journal of Bacteriology, August 1998, p. 4233-4242, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Wang, Y., Lechno-Yossef, S., Gong, Y., Fan, Q., Wolk, C. P., Xu, X.
(2007). Predicted Glycosyl Transferase Genes Located outside the HEP Island Are Required for Formation of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120. J. Bacteriol.
189: 5372-5378
[Abstract]
[Full Text]
-
Lechno-Yossef, S., Fan, Q., Ehira, S., Sato, N., Wolk, C. P.
(2006). Mutations in Four Regulatory Genes Have Interrelated Effects on Heterocyst Maturation in Anabaena sp. Strain PCC 7120. J. Bacteriol.
188: 7387-7395
[Abstract]
[Full Text]
-
Fan, Q., Lechno-Yossef, S., Ehira, S., Kaneko, T., Ohmori, M., Sato, N., Tabata, S., Wolk, C. P.
(2006). Signal Transduction Genes Required for Heterocyst Maturation in Anabaena sp. Strain PCC 7120.. J. Bacteriol.
188: 6688-6693
[Abstract]
[Full Text]
-
Sakr, S., Jeanjean, R., Zhang, C.-C., Arcondeguy, T.
(2006). Inhibition of Cell Division Suppresses Heterocyst Development in Anabaena sp. Strain PCC 7120. J. Bacteriol.
188: 1396-1404
[Abstract]
[Full Text]
-
Wang, Y., Xu, X.
(2005). Regulation by hetC of Genes Required for Heterocyst Differentiation and Cell Division in Anabaena sp. Strain PCC 7120. J. Bacteriol.
187: 8489-8493
[Abstract]
[Full Text]
-
Ramirez, M. E., Hebbar, P. B., Zhou, R., Wolk, C. P., Curtis, S. E.
(2005). Anabaena sp. Strain PCC 7120 Gene devH Is Required for Synthesis of the Heterocyst Glycolipid Layer. J. Bacteriol.
187: 2326-2331
[Abstract]
[Full Text]
-
Huang, G., Fan, Q., Lechno-Yossef, S., Wojciuch, E., Wolk, C. P., Kaneko, T., Tabata, S.
(2005). Clustered Genes Required for the Synthesis of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120. J. Bacteriol.
187: 1114-1123
[Abstract]
[Full Text]
-
Wu, X., Liu, D., Lee, M. H., Golden, J. W.
(2004). patS Minigenes Inhibit Heterocyst Development of Anabaena sp. Strain PCC 7120. J. Bacteriol.
186: 6422-6429
[Abstract]
[Full Text]
-
Huang, X., Dong, Y., Zhao, J.
(2004). HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS. Proc. Natl. Acad. Sci. USA
101: 4848-4853
[Abstract]
[Full Text]
-
Ning, D., Xu, X.
(2004). alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp. PCC 7120. Microbiology
150: 447-453
[Abstract]
[Full Text]
-
Zhou, R., Wolk, C. P.
(2003). A Two-component System Mediates Developmental Regulation of Biosynthesis of a Heterocyst Polysaccharide. J. Biol. Chem.
278: 19939-19946
[Abstract]
[Full Text]
-
Liu, D., Golden, J. W.
(2002). hetL Overexpression Stimulates Heterocyst Formation in Anabaena sp. Strain PCC 7120. J. Bacteriol.
184: 6873-6881
[Abstract]
[Full Text]
-
Koksharova, O. A., Wolk, C. P.
(2002). Novel DNA-Binding Proteins in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol.
184: 3931-3940
[Abstract]
[Full Text]
-
Zhu, J., Jager, K., Black, T., Zarka, K., Koksharova, O., Wolk, C. P.
(2001). HcwA, an Autolysin, Is Required for Heterocyst Maturation in Anabaena sp. Strain PCC 7120. J. Bacteriol.
183: 6841-6851
[Abstract]
[Full Text]
-
Yoon, H.-S., Golden, J. W.
(2001). PatS and Products of Nitrogen Fixation Control Heterocyst Pattern. J. Bacteriol.
183: 2605-2613
[Abstract]
[Full Text]
-
Hagen, K. D., Meeks, J. C.
(1999). Biochemical and Genetic Evidence for Participation of DevR in a Phosphorelay Signal Transduction Pathway Essential for Heterocyst Maturation in Nostoc punctiforme ATCC 29133. J. Bacteriol.
181: 4430-4434
[Abstract]
[Full Text]
-
Schwartz, S. H., Black, T. A., Jäger, K., Panoff, J.-M., Wolk, C. P.
(1998). Regulation of an Osmoticum-Responsive Gene in Anabaena sp. Strain PCC 7120. J. Bacteriol.
180: 6332-6337
[Abstract]
[Full Text]