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Journal of Bacteriology, August 1998, p. 4233-4242, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Regulation of hepA of Anabaena sp. Strain PCC 7120 by Elements 5' from the Gene and by hepK

Jinsong Zhu,dagger Renqiu Kong,Dagger and C. Peter Wolk*

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

Received 27 March 1998/Accepted 4 June 1998

In Anabaena spp., synthesis of the heterocyst envelope polysaccharide, required if the cell is to fix dinitrogen under aerobic conditions, is dependent on the gene hepA. A transcriptional start site of hepA was localized 104 bp 5' from its translational initiation codon. A 765-bp open reading frame, denoted hepC, was found farther upstream. Inactivation of hepC led to constitutive expression of hepA and prevented the synthesis of heterocyst envelope polysaccharide. However, the glycolipid layer of the heterocyst envelope was synthesized. A hepK mutation blocked both the synthesis of the heterocyst envelope polysaccharide and induction of hepA. The predicted product of hepK resembles a sensory protein-histidine kinase of a two-component regulatory system. Analysis of the region between hepC and hepA indicated that DNA sequences required for the induction of hepA upon nitrogen deprivation are present between bp -574 and -440 and between bp -340 and -169 relative to the transcriptional start site of hepA. Gel mobility shift assays provided evidence that one or more proteins bind specifically to the latter sequence. The Fox box sequence downstream from hepA appeared inessential for the induction of hepA.

* Corresponding author. Mailing address: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824. Phone: (517) 353-2049. Fax: (517) 353-9168. E-mail: wolk{at}pilot.msu.edu.

dagger Present address: Department of Entomology, Michigan State University, East Lansing, MI 48824.

Dagger Present address: Department of Microbiology, Michigan State University, East Lansing, MI 48824.

Journal of Bacteriology, August 1998, p. 4233-4242, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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