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Journal of Bacteriology, August 1998, p. 4252-4257, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Localization and Function of Early Cell
Division Proteins in Filamentous Escherichia coli Cells
Lacking Phosphatidylethanolamine
Eugenia
Mileykovskaya,1
Qin
Sun,2
William
Margolin,2 and
William
Dowhan1,*
Department of Biochemistry and Molecular
Biology1 and
Department of
Microbiology and Molecular Genetics,2
University of Texas
Houston, Medical School, Houston, Texas 77225
Received 5 March 1998/Accepted 12 June 1998
Escherichia coli cells that contain the
pss-93 null mutation are completely deficient in the major
membrane phospholipid phosphatidylethanolamine (PE). Such cells are
defective in cell division. To gain insight into how a phospholipid
defect could block cytokinesis, we used fluorescence techniques on
whole cells to investigate which step of the cell division cycle was
affected. Several proteins essential for early steps in cytokinesis,
such as FtsZ, ZipA, and FtsA, were able to localize as bands to
potential division sites in pss-93 filaments, indicating
that the generation and localization of potential division sites was
not grossly affected by the absence of PE. However, there was no
evidence of constriction at most of these potential division sites.
FtsZ and green fluorescent protein (GFP) fusions to FtsZ and ZipA often
formed spiral structures in these mutant filaments. This is the first
report of spirals formed by wild-type FtsZ expressed at normal levels
and by ZipA-GFP. The results suggest that the lack of PE may affect the
correct interaction of FtsZ with membrane nucleation sites and alter
FtsZ ring structure so as to prevent or delay its constriction.
*
Corresponding author. Mailing address: Department of
Biochemistry and Molecular Biology, University of Texas
Houston,
Medical School, P.O. Box 20708, Houston, TX 77225. Phone: (713)
500-6051. Fax: (713) 500-0652. E-mail:
wdowhan{at}utmmg.med.uth.tmc.edu.
Journal of Bacteriology, August 1998, p. 4252-4257, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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