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Journal of Bacteriology, August 1998, p. 4294-4299, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Involvement of the gapA- and epd (gapB)-Encoded Dehydrogenases in Pyridoxal 5'-Phosphate Coenzyme Biosynthesis in Escherichia coli K-12

Yong Yang, Genshi Zhao,dagger Tsz-Kwong Man, and Malcolm E. Winkler*

Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030-1501

Received 9 April 1998/Accepted 12 June 1998

We show that epd (gapB) mutants lacking an erythrose 4-phosphate (E4P) dehydrogenase are impaired for growth on some media and contain less pyridoxal 5'-phosphate (PLP) and pyridoxamine 5'-phosphate (PMP) than their epd+ parent. In contrast to a previous report, we found that gapA epd double mutants lacking the glyceraldehyde 3-phosphate and E4P dehydrogenases are auxotrophic for pyridoxine. These results implicate the GapA and Epd dehydrogenases in de novo PLP and PMP coenzyme biosynthesis.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 6431 Fannin; JFB 1.765, Houston, TX 77030-1501. Phone: (713) 500-5461. Fax: (713) 500-5499. E-mail: mwinkler{at}utmmg.med.uth.tmc.edu.

dagger Present address: Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285-0438.

Journal of Bacteriology, August 1998, p. 4294-4299, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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