Journal of Bacteriology, August 1998, p. 4300-4302, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392
Received 7 April 1998/Accepted 4 June 1998
Wild-type VirE2 and VirD2 proteins from Agrobacterium
tumefaciens contain nuclear targeting sequences (NLS) that are
likely involved in directing transferred T strands to the plant
nucleus. An A. tumefaciens virE2 virD2
NLS double
mutant was able to form tumors on VirE2-producing transgenic tobacco
but not on wild-type tobacco. Because this mutant bacterial strain
contains no known T-strand nuclear targeting signal, the data indicate
that wild-type VirE2 proteins produced by the plant can interact with
the T strands in the plant cytoplasm and direct them to the nucleus.
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