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Journal of Bacteriology, August 1998, p. 4319-4323, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of the Genes Encoding D-Amino Acid Transaminase and Glutamate Racemase, Two D-Glutamate Biosynthetic Enzymes of Bacillus sphaericus ATCC 10208

Ian G. Fotheringham,* Stefan A. Bledig, and Paul P. Taylor

Biosciences Laboratory, NSC Technologies, Mt. Prospect, Illinois 60056

Received 3 April 1998/Accepted 27 May 1998

In Bacillus sphaericus and other Bacillus spp., D-amino acid transaminase has been considered solely responsible for biosynthesis of D-glutamate, an essential component of cell wall peptidoglycan, in contrast to the glutamate racemase employed by many other bacteria. We report here the cloning of the dat gene encoding D-amino acid transaminase and the glr gene encoding a glutamate racemase from B. sphaericus ATCC 10208. The glr gene encodes a 28.8-kDa protein with 40 to 50% sequence identity to the glutamate racemases of Lactobacillus, Pediococcus, and Staphylococcus species. The dat gene encodes a 31.4-kDa peptide with 67% primary sequence homology to the D-amino acid transaminase of the thermophilic Bacillus sp. strain YM1.

* Corresponding author. Mailing address: Biosciences Laboratory, NSC Technologies, 601 East Kensington Rd., Mt. Prospect, IL 60056. Phone: (847) 506-2839. Fax: (847) 506-4270. E-mail: igfoth{at}ccmail.monsanto.com.

Journal of Bacteriology, August 1998, p. 4319-4323, Vol. 180, No. 16
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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