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Journal of Bacteriology, September 1998, p. 4380-4386, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
The carB Gene Encoding the Large Subunit of
Carbamoylphosphate Synthetase from Lactococcus
lactis Is Transcribed Monocistronically
Jan
Martinussen* and
Karin
Hammer
Department of Microbiology, The Technical
University of Denmark, DK-2800 Lyngby, Denmark
Received 14 January 1998/Accepted 23 June 1998
The biosynthesis of carbamoylphosphate is catalyzed by
the heterodimeric enzyme carbamoylphosphate synthetase. The
genes encoding the two subunits of this enzyme in procaryotes are
normally transcribed as an operon, but the gene encoding the
large subunit (carB) in Lactococcus lactis is
shown to be transcribed as an isolated unit. Carbamoylphosphate is a precursor in the biosynthesis of
both pyrimidine nucleotides and arginine. By mutant analysis,
L. lactis is shown to possess only one
carB gene; the same gene product is thus
required for both biosynthetic pathways. Furthermore, arginine may satisfy the requirement for
carbamoylphosphate in pyrimidine biosynthesis through
degradation by means of the arginine deiminase pathway. The expression
of the carB gene is subject to regulation at the
level of transcription by pyrimidines, most probably by an
attenuator mechanism. Upstream of the carB gene, an
open reading frame showing a high degree of similarity to those of
glutathione peroxidases from other organisms was identified.
*
Corresponding author. Mailing address: Department of
Microbiology, Building 301, The Technical University of Denmark,
DK-2800 Lyngby, Denmark. Phone: 45 45 25 24 94. Fax: 45 45 88 26 60. E-mail: jm{at}im.dtu.dk.
Journal of Bacteriology, September 1998, p. 4380-4386, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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