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Journal of Bacteriology, September 1998, p. 4667-4675, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Novel Pathway for Conversion of Chlorohydroxyquinol to Maleylacetate in Burkholderia cepacia AC1100

Olga Zaborina, Dayna L. Daubaras,dagger Anna Zago, Luying Xun,Dagger Katsuhiko Saido,§ Thomas Klem, Dejan Nikolic,parallel and A. M. Chakrabarty*

Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612

Received 1 April 1998/Accepted 8 June 1998

Burkholderia cepacia AC1100 metabolizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) via formation of 5-chlorohydroxyquinol (5-CHQ), hydroxyquinol (HQ), maleylacetate, and beta -oxoadipate. The step(s) leading to the dechlorination of 5-CHQ to HQ has remained unidentified. We demonstrate that a dechlorinating enzyme, TftG, catalyzes the conversion of 5-CHQ to hydroxybenzoquinone, which is then reduced to HQ by a hydroxybenzoquinone reductase (HBQ reductase). HQ is subsequently converted to maleylacetate by hydroxyquinol 1,2-dioxygenase (HQDO). All three enzymes were purified. We demonstrate specific product formation by colorimetric assay and mass spectrometry when 5-CHQ is treated successively with the three enzymes: TftG, TftG plus HBQ reductase, and TftG plus HBQ reductase plus HQDO. This study delineates the complete enzymatic pathway for the degradation of 5-CHQ to maleylacetate.

* Corresponding author. Mailing address: Department of Microbiology and Immunology (M/C 790), College of Medicine, University of Illinois at Chicago, 835 South Wolcott, Chicago, IL 60612. Phone: (312) 996-4586. Fax: (312) 996-6415. E-mail: Ananda.Chakrabarty{at}uic.edu.

dagger Present address: Schering-Plough Research Institute, Kenilworth, NJ 07033.

Dagger Present address: Department of Microbiology, Washington State University, Pullman, WA 99164.

§ College of Pharmacy, Nihon University, Chiba 274, Japan.

parallel Present address: College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612.

Journal of Bacteriology, September 1998, p. 4667-4675, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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