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Journal of Bacteriology, September 1998, p. 4734-4738, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning, Expression, and Catabolite Repression of a Gene Encoding beta -Galactosidase of Bacillus megaterium ATCC 14581

Gwo-Chyuan Shaw,* Hsun-Sheng Kao, and Chih-Yung Chiou

Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan, Republic of China

Received 5 May 1998/Accepted 17 June 1998

A gene encoding beta -galactosidase, designated mbgA, was isolated from Bacillus megaterium ATCC 14581. Chromosomal beta -galactosidase production could be dramatically induced by lactose but not by isopropyl-beta -D-thiogalactopyranoside (IPTG) and was subject to catabolite repression by glucose. Disruption of mbgA in the B. megaterium chromosome resulted in loss of lactose-inducible beta -galactosidase production. A 27-bp inverted repeat was found to overlap the mbgA promoter sequence. Two partially overlapping catabolite-responsive elements (CREs) were identified within the inverted repeat. Base substitutions within CRE-I and/or CRE-II caused partial relief from catabolite repression. The results suggest that the 27-bp inverted repeat may serve as a target for a catabolite repressor(s).

* Corresponding author. Mailing address: Institute of Biochemistry, School of Life Science, National Yang-Ming University, Taipei, Taiwan 112, Republic of China. Phone: 886-2-2826-7127. Fax: 886-2-2826-4843. E-mail: gcshaw{at}mailsrv.ym.edu.tw.

Journal of Bacteriology, September 1998, p. 4734-4738, Vol. 180, No. 17
0021-9193/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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